Identification of a novel inducible cell-surface ligand of CD5 on activated lymphocytes.

Details

Serval ID
serval:BIB_B87C6904966A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of a novel inducible cell-surface ligand of CD5 on activated lymphocytes.
Journal
Journal of Experimental Medicine
Author(s)
Biancone L., Bowen M.A., Lim A., Aruffo A., Andres G., Stamenkovic I.
ISSN
0022-1007[print], 0022-1007[linking]
Publication state
Published
Issued date
1996
Volume
184
Number
3
Pages
811-819
Language
english
Abstract
CD5 is a 67-kD glycoprotein that is expressed on most T lymphocytes and on a subset of mature B cells. Although its physiologic function is unknown, several lines of evidence suggest that CD5 may play a role in the regulation of T cell activation and in T cell-antigen presenting cell interactions. Using a CD5-immunoglobulin fusion protein (CD5Rg, for receptorglobulin) we have uncovered a new CD5 ligand (CD5L) expressed on the surface of activated splenocytes. Stimulation of murine splenocytes with anti-CD3 and anti-CD28 antibodies induce transient expression of CD5L on B lymphocytes that lasts for approximately 72 h. Binding of CD5Rg to activated splenocytes is trypsin resistant and independent of divalent cations. However, it is pronase sensitive and dependent on N-linked glycosylation of CD5, since treatment of CD5Rg with PNGaseF on N-glycanase completely abrogates its ability to bind activated splenocytes. It addition to splenocytes, CD5L is expressed on activated murine T cell clones. Immunoprecipitation, antibody, and recombinant protein blocking studies indicate that CD5L is distinct from CD72, which has been proposed to be a CD5 ligand. To determine whether CD5-CD5L interaction might play a role in vivo, we tested the effect of CD5Rg in a murine model of antibody-mediated membranous glomerulonephritis. Injection of CD5Rg was found to abrogate development of the disease. Taken together, our results help identify a novel ligand of CD5 and propose a role for CD5 in the regulation of immune responses.
Keywords
Animals, Antigens, CD5/analysis, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Glomerulonephritis, Membranous/prevention & control, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Receptors, Cell Surface/analysis, Spectrometry, Fluorescence, Spleen/cytology, Spleen/metabolism
Pubmed
Create date
26/08/2010 16:44
Last modification date
20/08/2019 15:26
Usage data