Keratinocyte growth factor increases transalveolar sodium reabsorption in normal and injured rat lungs

Details

Serval ID
serval:BIB_B7C498A6B33B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Keratinocyte growth factor increases transalveolar sodium reabsorption in normal and injured rat lungs
Journal
Am J Respir Crit Care Med
Author(s)
Guery B. P., Mason C. M., Dobard E. P., Beaucaire G., Summer W. R., Nelson S.
ISSN
1073-449X (Print)
ISSN-L
1073-449X
Publication state
Published
Issued date
05/1997
Volume
155
Number
5
Pages
1777-84
Language
english
Notes
Guery, B P
Mason, C M
Dobard, E P
Beaucaire, G
Summer, W R
Nelson, S
eng
AA09803/AA/NIAAA NIH HHS/
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Am J Respir Crit Care Med. 1997 May;155(5):1777-84. doi: 10.1164/ajrccm.155.5.9154891.
Abstract
Keratinocyte growth factor (KGF) prevents alpha-naphthylthiourea (ANTU)-induced permeability edema ex vivo. To explore the mechanisms in this involved effect, we administered KGF (5 mg/kg, intratracheally) 48 h prior to ANTU (50 mg/kg, intraperitoneally). Several groups were studied: phosphate-buffered saline/dimethylsulfoxide (PBS/DMSO) (vehicles), PBS/ANTU, and KGF/ANTU. At 90 min after ANTU injection the lungs were removed, ventilated, and perfused ex vivo for 180 min. Quantification of fluorescein isothiocyanate (FITC)-labeled dextran in bronchoalveolar lavage fluid (BALF) was used to assess alveolar capillary barrier permeability. KGF attenuated ANTU-induced edema and blockade of sodium transport, with ouabain (10(-3) M) or amiloride (10(-4) M) added ex vivo reversed this effect. FITC-dextran was increased in the PBS/ANTU group as compared with the PBS/DMSO group, indicating permeability edema. In the KGF/ANTU group, there was concentration of BALF FITC-dextran, consistent with permeability edema and increased alveolar fluid export. Albumin space measurements showed similar increases in permeability in the PBS/ANTU and KGF/ANTU groups. Extravascular lung water (measured with radiolabeled erythrocytes) was decreased in the KGF/ANTU group. Following KGF pretreatment, uninjured lungs exported more intratracheal PBS than normal lungs following terbutaline stimulation ex vivo. In conclusion, KGF, through type II alveolar pneumocyte hyperplasia with increased sodium-potassium-adenosine triphosphatase (Na,K-ATPase) activity, attenuated ANTU-induced edema formation by potentiating alveolar fluid clearance.
Keywords
Absorption, Amiloride/pharmacology, Animals, Biological Transport, Bronchoalveolar Lavage Fluid/chemistry, Capillary Permeability, Dimethyl Sulfoxide/pharmacology, Enzyme Activation, Extravascular Lung Water/metabolism, Fibroblast Growth Factor 10, Fibroblast Growth Factor 7, *Fibroblast Growth Factors, Growth Substances/*pharmacology, Ouabain/pharmacology, Pulmonary Alveoli/*metabolism/pathology, Pulmonary Edema/chemically induced/*metabolism/pathology/physiopathology, Rats, Sodium/*metabolism, Sodium Chloride, Sodium-Potassium-Exchanging ATPase/metabolism, Thiourea/analogs & derivatives
Pubmed
Create date
29/04/2021 9:59
Last modification date
30/04/2021 5:38
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