Combinatorial peptide library-based identification of peptide ligands for tumor-reactive cytolytic T lymphocytes of unknown specificity.

Details

Serval ID
serval:BIB_B7AC147FA5C1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Combinatorial peptide library-based identification of peptide ligands for tumor-reactive cytolytic T lymphocytes of unknown specificity.
Journal
European Journal of Immunology
Author(s)
Rubio-Godoy V., Ayyoub M., Dutoit V., Servis C., Schink A., Rimoldi D., Romero P., Cerottini J.C., Simon R., Zhao Y., Houghten R.A., Pinilla C., Valmori D.
ISSN
0014-2980, 0014-2980[linking]
Publication state
Published
Issued date
2002
Volume
32
Number
8
Pages
2292-2299
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
A novel approach for the identification of tumor antigen-derived sequences recognized by CD8(+) cytolytic T lymphocytes (CTL) consists in using synthetic combinatorial peptide libraries. Here we have screened a library composed of 3.1 x 10(11) nonapeptides arranged in a positional scanning format, in a cytotoxicity assay, to search the antigen recognized by melanoma-reactive CTL of unknown specificity. The results of this analysis enabled the identification of several optimal peptide ligands, as most of the individual nonapeptides deduced from the primary screening were efficiently recognized by the CTL. The results of the library screening were also analyzed with a mathematical approach based on a model of independent and additive contribution of individual amino acids to antigen recognition. This biometrical data analysis enabled the retrieval, in public databases, of the native antigenic peptide SSX-2(41-49), whose sequence is highly homologous to the ones deduced from the library screening, among the ones with the highest stimulatory score. These results underline the high predictive value of positional scanning synthetic combinatorial peptide library analysis and encourage its use for the identification of CTL ligands.
Keywords
Antigens, Neoplasm/immunology, Cell Line, Combinatorial Chemistry Techniques, Humans, Ligands, Peptide Library, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:14
Last modification date
20/08/2019 15:25
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