Effect of two types of biosurfactants on phenanthrene availability to the bacterial bioreporter Burkholderia sartisoli strain RP037.

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Serval ID
serval:BIB_B7567FDB5CBA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effect of two types of biosurfactants on phenanthrene availability to the bacterial bioreporter Burkholderia sartisoli strain RP037.
Journal
Applied microbiology and biotechnology
Author(s)
Tecon R., van der Meer J.R.
ISSN
1432-0614[electronic]
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
85
Number
4
Pages
1131-1139
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Biosurfactants are tensio-active agents that have often been proposed as a means to enhance the aqueous solubility of hydrophobic organic contaminants, such as polycyclic aromatic hydrocarbons (PAHs). Biosurfactant-producing bacteria such as those belonging to the genus Pseudomonas might therefore enhance PAH availability to PAH-degrading bacteria. We tested the effects of two types of biosurfactants produced by Pseudomonas sp., cyclic lipopeptides and rhamnolipids, on phenanthrene bioavailability. Bioavailability was judged from growth rates on phenanthrene and from specific induction of a phenanthrene-responsive GFP-reporter in Burkholderia sartisoli strain RP037. Co-culturing of strain RP037 with the lipopeptide-producing bacterium Pseudomonas putida strain PCL1445 enhanced GFP expression compared to a single culture, but this effect was not significantly different when strain RP037 was co-cultivated with a non-lipopeptide-producing mutant of P. putida. The addition of partially purified supernatant extracts from the P. putida lipopeptide producer equally did not unequivocally enhance phenanthrene bioavailability to strain RP037 compared to controls. In contrast, a 0.1% rhamnolipid solution strongly augmented RP037 growth rates on phenanthrene and led to a significantly larger proportion of cells in culture with high GFP expression. Our data therefore suggest that biosurfactant effects may be strongly dependent on the strain and type of biosurfactant.
Keywords
Bioreporter, GFP, Rhamnolipid, Lipopeptide, Polycyclic aromatic hydrocarbons (PAH)
Pubmed
Web of science
Open Access
Yes
Create date
13/01/2010 13:31
Last modification date
01/10/2019 6:19
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