Differential production of systemic and intralesional gamma interferon and interleukin-10 in nodular and ulcerative forms of Buruli disease.

Details

Serval ID
serval:BIB_B7542E02FF4C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Differential production of systemic and intralesional gamma interferon and interleukin-10 in nodular and ulcerative forms of Buruli disease.
Journal
Infection and Immunity
Author(s)
Prévot G., Bourreau E., Pascalis H., Pradinaud R., Tanghe A., Huygen K., Launois P.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
2004
Volume
72
Number
2
Pages
958-965
Language
english
Abstract
Buruli disease, caused by Mycobacterium ulcerans, is the third most important mycobacterial disease in humans besides tuberculosis and leprosy. We have compared systemic and intralesional cytokine production in patients presenting with a nodular form and a necrotizing, ulcerative form of the disease. Gamma interferon (IFN-gamma) levels in response to whole M. ulcerans and Mycobacterium bovis BCG bacilli and in response to purified Ag85 protein from BCG were lower in peripheral blood mononuclear cells (PBMC) cultures from Buruli disease patients than in PBMC from healthy purified protein derivative-positive contacts. Interleukin-4 (IL-4) and IL-13 content was below the detection threshold in these PBMC cultures. IFN-gamma production after stimulation with M. ulcerans was significantly lower (P < 0.05) in PBMC cultures from patients with ulcers than in those from patients with nodules. On the other hand, PBMC from Buruli disease patients produced significant levels of IL-10 in response to M. ulcerans (but not to M. bovis BCG) and production was highest in patients with the ulcerative form. Third, semiquantitative reverse transcription-PCR analysis demonstrated a similar difference in the local, intralesional cytokine profile for the two forms of the disease: high IFN-gamma but low IL-10 mRNA levels in nodular lesions and high IL-10 but low IFN-gamma mRNA levels in ulcerative lesions. Intralesional IL-4 and IL-13 mRNA levels were low and only detected in patients with the ulcerative form. Our results indicate, although they do not formally prove, that production of IL-10 rather than production of IL-4 or IL-13 by Th2-type T cells may be involved in the low M. ulcerans-specific IFN-gamma response in Buruli disease patients.
Keywords
Adolescent, Adult, Child, Preschool, Female, Humans, Immune Tolerance, Interferon-gamma/biosynthesis, Interleukin-10/biosynthesis, Interleukin-4/biosynthesis, Male, Middle Aged, Mycobacterium Infections, Atypical/immunology, Mycobacterium ulcerans, Skin/immunology, Skin Ulcer/immunology, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:06
Last modification date
20/08/2019 15:25
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