BactMAP: An R package for integrating, analyzing and visualizing bacterial microscopy data.

Details

Serval ID
serval:BIB_B71D72E12E04
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
BactMAP: An R package for integrating, analyzing and visualizing bacterial microscopy data.
Journal
Molecular microbiology
Author(s)
van Raaphorst R., Kjos M., Veening J.W.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Publication state
Published
Issued date
01/2020
Peer-reviewed
Oui
Volume
113
Number
1
Pages
297-308
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
High-throughput analyses of single-cell microscopy data are a critical tool within the field of bacterial cell biology. Several programs have been developed to specifically segment bacterial cells from phase-contrast images. Together with spot and object detection algorithms, these programs offer powerful approaches to quantify observations from microscopy data, ranging from cell-to-cell genealogy to localization and movement of proteins. Most segmentation programs contain specific post-processing and plotting options, but these options vary between programs and possibilities to optimize or alter the outputs are often limited. Therefore, we developed BactMAP (Bacterial toolbox for Microscopy Analysis & Plotting), a command-line based R package that allows researchers to transform cell segmentation and spot detection data generated by different programs into various plots. Furthermore, BactMAP makes it possible to perform custom analyses and change the layout of the output. Because BactMAP works independently of segmentation and detection programs, inputs from different sources can be compared within the same analysis pipeline. BactMAP complies with standard practice in R which enables the use of advanced statistical analysis tools, and its graphic output is compatible with ggplot2, enabling adjustable plot graphics in every operating system. User feedback will be used to create a fully automated Graphical User Interface version of BactMAP in the future. Using BactMAP, we visualize key cell cycle parameters in Bacillus subtilis and Staphylococcus aureus, and demonstrate that the DNA replication forks in Streptococcus pneumoniae dissociate and associate before splitting of the cell, after the Z-ring is formed at the new quarter positions. BactMAP is available from https://veeninglab.com/bactmap.
Keywords
Bacillus subtilis, Staphylococcus aureus, Streptococcus pneumoniae, Bacterial cell biology, Chromosome segregation, DNA replication, Image analysis, Rtools, Single cell analysis, bacterial cell biology, chromosome segregation, image analysis, single cell analysis, Bacillus subtilis, Staphylococcus aureus, Streptococcus pneumoniae
Pubmed
Web of science
Open Access
Yes
Create date
07/11/2019 23:24
Last modification date
18/02/2020 7:20
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