Camphor and Eucalyptol-Anticandidal Spectrum, Antivirulence Effect, Efflux Pumps Interference and Cytotoxicity.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_B7158D5FEC90
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Camphor and Eucalyptol-Anticandidal Spectrum, Antivirulence Effect, Efflux Pumps Interference and Cytotoxicity.
Journal
International journal of molecular sciences
Author(s)
Ivanov M., Kannan A., Stojković D.S., Glamočlija J., Calhelha R.C., Ferreira ICFR, Sanglard D., Soković M.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
06/01/2021
Peer-reviewed
Oui
Volume
22
Number
2
Pages
483
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Candidaalbicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125-0.35 mg/mL while eucalyptol was active in the range of 2-23 mg/mL. The results showed camphor's potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1;CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.
Keywords
antifungal activity, camphor, cytotoxicity, efflux pumps, eucalyptol, terpenoids, virulence factors
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2021 11:26
Last modification date
05/10/2021 5:40
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