Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections

Details

Serval ID
serval:BIB_B6937D53A3EF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections
Journal
Environ Microbiol
Author(s)
Aragon I. M., Perez-Mendoza D., Moscoso J. A., Faure E., Guery B., Gallegos M. T., Filloux A., Ramos C.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Publication state
Published
Issued date
11/2015
Volume
17
Number
11
Pages
4332-51
Language
english
Notes
Aragon, Isabel M
Perez-Mendoza, Daniel
Moscoso, Joana A
Faure, Emmanuel
Guery, Benoit
Gallegos, Maria-Trinidad
Filloux, Alain
Ramos, Cayo
eng
MR/J006874/1/Medical Research Council/United Kingdom
BB/L007959/1/Biotechnology and Biological Sciences Research Council/United Kingdom
Research Support, Non-U.S. Gov't
England
Environ Microbiol. 2015 Nov;17(11):4332-51. doi: 10.1111/1462-2920.12856. Epub 2015 Apr 28.
Abstract
The second messenger cyclic di-GMP (c-di-GMP) controls the transition between different lifestyles in bacterial pathogens. Here, we report the identification of DgcP (diguanylate cyclase conserved in Pseudomonads), whose activity in the olive tree pathogen Pseudomonas savastanoi pv. savastanoi is dependent on the integrity of its GGDEF domain. Furthermore, deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa. Overexpression of the dgcP gene in P. aeruginosa PAK led to increased exopolysaccharide production and upregulation of the type VI secretion system; in turn, it repressed the type III secretion system, which is a hallmark of chronic infections and persistence for P. aeruginosa. Deletion of the dgcP gene in P. savastanoi pv. savastanoi NCPPB 3335 and P. aeruginosa PAK reduced their virulence in olive plants and in a mouse acute lung injury model respectively. Our results show that diguanylate cyclase DgcP is a conserved Pseudomonas protein with a role in virulence, and confirm the existence of common c-di-GMP signalling pathways that are capable of regulating plant and human Pseudomonas spp. infections.
Keywords
Acute Lung Injury/*microbiology, Animals, Biofilms/growth & development, Cyclic GMP/analogs & derivatives/metabolism, Disease Models, Animal, Escherichia coli Proteins/*genetics, Humans, Mice, Olea/microbiology, Phosphorus-Oxygen Lyases/*genetics, Plant Diseases/*microbiology, Protein Structure, Tertiary, Pseudomonas Infections/*microbiology, Pseudomonas aeruginosa/*genetics/*pathogenicity, Sequence Deletion, Signal Transduction/genetics, Type III Secretion Systems/genetics/metabolism, Type VI Secretion Systems/genetics/metabolism, Virulence/genetics
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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