The human NAIP-NLRC4-inflammasome senses the Pseudomonas aeruginosa T3SS inner-rod protein.

Details

Serval ID
serval:BIB_B66F2575F681
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The human NAIP-NLRC4-inflammasome senses the Pseudomonas aeruginosa T3SS inner-rod protein.
Journal
International immunology
Author(s)
Grandjean T., Boucher A., Thepaut M., Monlezun L., Guery B., Faudry E., Kipnis E., Dessein R.
ISSN
1460-2377 (Electronic)
ISSN-L
0953-8178
Publication state
Published
Issued date
01/08/2017
Peer-reviewed
Oui
Volume
29
Number
8
Pages
377-384
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
While NLRC4-dependent sensing of intracellular Gram-negative pathogens such as Salmonella enterica serovar typhimurium is a beneficial host response, NLRC4-dependent sensing of the Pseudomonas aeruginosa type 3 secretion system (T3SS) has been shown to be involved in pathogenicity. In mice, different pathogen-associated microbial patterns are sensed by the combination of the NLRC4-inflammasome with different neuronal apoptosis inhibitory proteins (NAIPs). NAIP2 is involved in sensing PscI, an inner-rod protein of the P. aeruginosa T3SS. Surprisingly, only a single human NAIP (hNAIP) has been found. Moreover, there is no description of hNAIP-NLRC4 inflammasome recognition of T3SS inner-rod proteins in humans. Here, we show that the P. aeruginosa T3SS inner-rod protein PscI and needle protein PscF are both sensed by the hNAIP-NLRC4 inflammasome in human macrophages and PBMCs from healthy donors, allowing caspase-1 and IL-1β maturation and resulting in a robust inflammatory response. TLR4 and TLR2 are involved in redundantly sensing these two T3SS components.
Keywords
Animals, CARD Signaling Adaptor Proteins/metabolism, Calcium-Binding Proteins/metabolism, Carrier Proteins/metabolism, Caspase 1/metabolism, Humans, Immunity, Innate, Inflammasomes/metabolism, Interleukin-1beta/metabolism, Macrophages/immunology, Macrophages/microbiology, Mice, Neuronal Apoptosis-Inhibitory Protein/metabolism, Pathogen-Associated Molecular Pattern Molecules/immunology, Pseudomonas Infections/immunology, Pseudomonas aeruginosa/immunology, THP-1 Cells, Toll-Like Receptor 2/metabolism, Toll-Like Receptor 4/metabolism, Type III Secretion Systems/immunology, Type III Secretion Systems/metabolism, Innate Immunity, Nod-like receptor, Pathogen-associated microbial pattern, Toll-like receptor
Pubmed
Web of science
Create date
19/10/2017 8:20
Last modification date
20/08/2019 15:24
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