MicroRNA Expression in the Aqueous Humor of Patients with Diabetic Macular Edema.

Details

Serval ID
serval:BIB_B62D92353664
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
MicroRNA Expression in the Aqueous Humor of Patients with Diabetic Macular Edema.
Journal
International journal of molecular sciences
Author(s)
Grieco G.E., Sebastiani G., Eandi C.M., Neri G., Nigi L., Brusco N., D'Aurizio R., Posarelli M., Bacci T., Benedetto E., Fruschelli M., Orlandini M., Galvagni F., Dotta F., Tosi G.M.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
03/10/2020
Peer-reviewed
Oui
Volume
21
Number
19
Pages
E7328
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
We identified and compared secreted microRNA (miRNA) expression in aqueous humor (AH) and plasma samples among patients with: type 2 diabetes mellitus (T2D) complicated by non-proliferative diabetic retinopathy (DR) associated with diabetic macular edema (DME) (DME group: 12 patients); T2D patients without DR (D group: 8 patients); and non-diabetic patients (CTR group: 10 patients). Individual patient AH samples from five subjects in each group were profiled on TaqMan Low Density MicroRNA Array Cards. Differentially expressed miRNAs identified from profiling were then validated in single assay for all subjects. The miRNAs validated in AH were then evaluated in single assay in plasma. Gene Ontology (GO) analysis was conducted. From AH profiling, 119 mature miRNAs were detected: 86 in the DME group, 113 in the D group and 107 in the CTR group. miRNA underexpression in the DME group was confirmed in single assay for let-7c-5p, miR-200b-3p, miR-199a-3p and miR-365-3p. Of these four, miR-199a-3p and miR-365-3p were downregulated also in the plasma of the DME group. GO highlighted 54 validated target genes of miR-199a-3p, miR-200b-3p and miR-365-3p potentially implied in DME pathogenesis. Although more studies are needed, miR-200b-3p, let-7c-5p, miR-365-3p and miR-199a-3p represent interesting molecules in the study of DME pathogenesis.
Keywords
Diabetic Macular Edema, aqueous humor, diabetic retinopathy, miRNA, microRNA, type 2 diabetes mellitus
Pubmed
Web of science
Open Access
Yes
Create date
09/10/2020 11:28
Last modification date
02/02/2021 6:26
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