High-resolution ultrasonography of subretinal structure and assessment of retina degeneration in rat.

Details

Serval ID
serval:BIB_B620DD9A1FB5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High-resolution ultrasonography of subretinal structure and assessment of retina degeneration in rat.
Journal
Experimental Eye Research
Author(s)
Jolly C., Jeanny J.C., Behar-Cohen F., Laugier P., Saïed A.
ISSN
0014-4835 (Print)
ISSN-L
0014-4835
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
81
Number
5
Pages
592-601
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
The purpose of this work was to evaluate the ability of 80 MHz ultrasonography to differentiate intra-retinal layers and quantitatively assess photoreceptor dystrophy in small animal models. Four groups of 10 RCS rats each (five dystrophic and five controls) were explored at 25, 35, 45 and 55 days post-natal (PN). A series of retina cross-sections were obtained ex vivo from outside intact eyes using an 80 MHz three-dimensional ultrasound backscatter microscope (20-microm-axial resolution). Ultrasound features of normal retina were correlated to those of corresponding histology and thickness measurements of photoreceptor segment and nuclear layers were performed on all groups. To show the ability of 80 MHz ultrasonography to distinguish the retinal degeneration in vivo, one RCS rat was explored at 25 and 55 days post-natal. Ultrasound image of normal retina displayed four distinct layers marked by reflections at neurites/nuclei interfaces and permitted to differentiate the photoreceptor segment and nuclear layers. The backscatter level from the retina was shown to be related to the size, density and organization of the intra-layer structure. Ultrasound thickness measurements highly correlated with histologic measurements. A thinning (p<0.05) of outer nuclear layer (ONL) was detected over time for controls and was thought to be assigned to retina maturation. Retinal degeneration started at PN35 and resulted in a more pronounced ONL thinning (p<0.05) over time. ONL degeneration was accompanied by segment layer thickening (p<0.05) at PN35 and thinning thereafter. These changes may indicate accumulation of outer segment debris at PN35 then progressive destruction. In vivo images of rat intra-retinal structure showed the ability of the method to distinguish the photoreceptor layer changes. Our results indicate that 80 MHz ultrasonography reveals intra-retinal layers and is sensitive to age and degenerative changes of photoreceptors. This technique has great potential to follow-up retinal dystrophy and therapeutic effects in vivo.
Keywords
Animals, Models, Animal, Photoreceptor Cells/ultrasonography, Rats, Rats, Inbred Strains, Retina/ultrasonography, Retinal Degeneration/ultrasonography, Sensitivity and Specificity
Pubmed
Web of science
Create date
08/11/2013 11:18
Last modification date
20/08/2019 15:24
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