Control of basal jasmonate signalling and defence through modulation of intracellular cation flux capacity.

Details

Serval ID
serval:BIB_B60AF2ED747A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Control of basal jasmonate signalling and defence through modulation of intracellular cation flux capacity.
Journal
The New Phytologist
Author(s)
Lenglet A., Jaślan D., Toyota M., Mueller M., Müller T., Schönknecht G., Marten I., Gilroy S., Hedrich R., Farmer E.E.
ISSN
1469-8137 (Electronic)
ISSN-L
0028-646X
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
216
Number
4
Pages
1161-1169
Language
english
Abstract
Unknown mechanisms tightly regulate the basal activity of the wound-inducible defence mediator jasmonate (JA) in undamaged tissues. However, the Arabidopsis fatty acid oxygenation upregulated2 (fou2) mutant in vacuolar two-pore channel 1 (TPC1(D454N) ) displays high JA pathway activity in undamaged leaves. This mutant was used to explore mechanisms controlling basal JA pathway regulation. fou2 was re-mutated to generate novel 'ouf' suppressor mutants. Patch-clamping was used to examine TPC1 cation channel characteristics in the ouf suppressor mutants and in fou2. Calcium (Ca(2+) ) imaging was used to study the effects fou2 on cytosolic Ca(2+) concentrations. Six intragenic ouf suppressors with near wild-type (WT) JA pathway activity were recovered and one mutant, ouf8, affected the channel pore. At low luminal calcium concentrations, ouf8 had little detectable effect on fou2. However, increased vacuolar Ca(2+) concentrations caused channel occlusion, selectively blocking K(+) fluxes towards the cytoplasm. Cytosolic Ca(2+) concentrations in unwounded fou2 were found to be lower than in the unwounded WT, but they increased in a similar manner in both genotypes following wounding. Basal JA pathway activity can be controlled solely by manipulating endomembrane cation flux capacities. We suggest that changes in endomembrane potential affect JA pathway activity.

Keywords
calcium, endomembrane, jasmonic acid (JA), membrane potential, two-pore channel 1 (TPC1)
Pubmed
Web of science
Open Access
Yes
Create date
23/11/2017 16:04
Last modification date
20/08/2019 15:24
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