Protein kinase A encoded by TPK2 regulates dimorphism of Candida albicans

Details

Serval ID
serval:BIB_B5FE3689D65C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Protein kinase A encoded by TPK2 regulates dimorphism of Candida albicans
Journal
Molecular Microbiology
Author(s)
Sonneborn  A., Bockmuhl  D. P., Gerads  M., Kurpanek  K., Sanglard  D., Ernst  J. F.
ISSN
0950-382X (Print)
Publication state
Published
Issued date
01/2000
Volume
35
Number
2
Pages
386-96
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
External signals induce the switch from a yeast to a hyphal growth form in the fungal pathogen Candida albicans. We demonstrate here that the catalytic subunit of a protein kinase A (PKA) isoform encoded by TPK2 is required for internal signalling leading to hyphal differentiation. TPK2 complements the growth defect of a Saccharomyces cerevisiae tpk1-3 mutant and Tpk2p is able to phosphorylate an established PKA-acceptor peptide (kemptide). Deletion of TPK2 blocks morphogenesis and partially reduces virulence, whereas TPK2 overexpression induces hyphal formation and stimulates agar invasion. The defective tpk2 phenotype is suppressed by overproduction of known signalling components, including Efg1p and Cek1p, whereas TPK2 overexpression reconstitutes the cek1 but not the efg1 phenotype. The results indicate that PKA activity of Tpk2p is an important contributing factor in regulating dimorphism of C. albicans.
Keywords
Animals Base Sequence Candida albicans/*enzymology/genetics/*growth & development/pathogenicity Candidiasis/microbiology/pathology Catalytic Domain Cloning, Molecular Culture Media Cyclic AMP-Dependent Protein Kinases/chemistry/genetics/*metabolism DNA, Complementary Epistasis, Genetic Gene Deletion Genes, Fungal Isoenzymes/chemistry/genetics/*metabolism Mice Molecular Sequence Data Morphogenesis Phenotype Plasmids/genetics Sequence Analysis, DNA Virulence
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:40
Last modification date
20/08/2019 15:24
Usage data