Inhibitory G protein alpha subunit (Gi alpha) expression and localization during human trophoblast differentiation.
Details
Serval ID
serval:BIB_B5BD7B25A2DD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inhibitory G protein alpha subunit (Gi alpha) expression and localization during human trophoblast differentiation.
Journal
European Journal of Obstetrics, Gynecology, and Reproductive Biology
ISSN
0301-2115 (Print)
ISSN-L
0301-2115
Publication state
Published
Issued date
2000
Volume
88
Number
2
Pages
165-169
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
OBJECTIVE: Cyclic adenosine monophosphate (cAMP) participates in the regulation of processes associated with trophoblast syncytialization. As guanosine triphosphate (GTP)-binding regulatory proteins (G-proteins) modulate adenylate cyclase activity, the present study investigated the expression and regulation of the alpha subunit of inhibitory G protein (Gi alpha) during human trophoblast differentiation in vitro.
STUDY DESIGN: Protein levels and the immunolocalization of the protein at a subcellular-level were assessed.
RESULTS: Expression of Gi alpha protein decreased during syncytialization. Moreover, Gi alpha transmigrated from a predominantly cell membrane-associated to a predominantly perinuclear location during the process.
CONCLUSION: These results suggest that Gi alpha is regulated and may be implicated in cAMP-dependent events during the terminal differentiation of human trophoblasts.
STUDY DESIGN: Protein levels and the immunolocalization of the protein at a subcellular-level were assessed.
RESULTS: Expression of Gi alpha protein decreased during syncytialization. Moreover, Gi alpha transmigrated from a predominantly cell membrane-associated to a predominantly perinuclear location during the process.
CONCLUSION: These results suggest that Gi alpha is regulated and may be implicated in cAMP-dependent events during the terminal differentiation of human trophoblasts.
Keywords
Adenosine Diphosphate Ribose/metabolism, Autoradiography, Cell Differentiation, Cell Membrane/metabolism, Cell Nucleus/metabolism, Culture Techniques, Fluorescent Antibody Technique, Indirect, GTP-Binding Protein alpha Subunits, Gi-Go/analysis, GTP-Binding Protein alpha Subunits, Gi-Go/metabolism, Humans, NAD/metabolism, Time Factors, Trophoblasts/chemistry, Trophoblasts/physiology
Pubmed
Web of science
Create date
14/10/2014 11:43
Last modification date
20/08/2019 15:24
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