Endothelial tip cells in vitro are less glycolytic and have a more flexible response to metabolic stress than non-tip cells.

Details

Serval ID
serval:BIB_B5A8078F702C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endothelial tip cells in vitro are less glycolytic and have a more flexible response to metabolic stress than non-tip cells.
Journal
Scientific reports
Author(s)
Yetkin-Arik B., Vogels IMC, Neyazi N., van Duinen V., Houtkooper R.H., van Noorden CJF, Klaassen I., Schlingemann R.O.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
18/07/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
10414
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Formation of new blood vessels by differentiated endothelial tip cells, stalk cells, and phalanx cells during angiogenesis is an energy-demanding process. How these specialized endothelial cell phenotypes generate their energy, and whether there are differences between these phenotypes, is unknown. This may be key to understand their functions, as (1) metabolic pathways are essentially involved in the regulation of angiogenesis, and (2) a metabolic switch has been associated with angiogenic endothelial cell differentiation. With the use of Seahorse flux analyses, we studied metabolic pathways in tip cell and non-tip cell human umbilical vein endothelial cell populations. Our study shows that both tip cells and non-tip cells use glycolysis as well as mitochondrial respiration for energy production. However, glycolysis is significantly lower in tip cells than in non-tip cells. Additionally, tip cells have a higher capacity to respond to metabolic stress. Finally, in non-tip cells, blocking of mitochondrial respiration inhibits endothelial cell proliferation. In conclusion, our data demonstrate that tip cells are less glycolytic than non-tip cells and that both endothelial cell phenotypes can adapt their metabolism depending on microenvironmental circumstances. Our results suggest that a balanced involvement of metabolic pathways is necessary for both endothelial cell phenotypes for proper functioning during angiogenesis.
Pubmed
Web of science
Open Access
Yes
Create date
02/08/2019 16:43
Last modification date
15/07/2020 5:26
Usage data