The major transcription initiation site of the SV40 late promoter is a potent thyroid hormone response element.

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Serval ID
serval:BIB_B58E25EE9F98
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The major transcription initiation site of the SV40 late promoter is a potent thyroid hormone response element.
Journal
Nucleic Acids Research
Author(s)
Desvergne B., Favez T.
ISSN
0305-1048[print], 0305-1048[linking]
Publication state
Published
Issued date
05/1997
Volume
25
Number
9
Pages
1774-1781
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Thyroid hormone receptors (TRs) are members of the nuclear hormone receptor superfamily, which act as transcription factors upon binding to specific DNA sequences called thyroid hormone (T3) response elements (TREs). Such elements are found in the upstream regulatory region of promoters as well as in intragenic sequences of T3-responsive genes. In this report, we demonstrate that SV40 late (SVL) promoter activity is strongly down-regulated by TR in the absence of ligand. Addition of T3 releases this repression, but does not further induce SVL promoter activity. Electrophoretic mobility shift analyses reveal a TR binding element that overlaps with the SV40 major late transcription initiation site. This element closely fits the consensus TRE, formed of two hexanucleotides organized in a tandem repeat separated by 4 nt, and is able to confer T3 responsiveness on a heterologous promoter. We further show that, although the presence of TR leads to quantitatively modified expression of an SVL-driven reporter gene, neither displacement of the site of transcription initiation nor modification of the splicing pattern of the primary transcripts occur.
Keywords
3T3 Cells, Animals, Base Sequence, Chloramphenicol O-Acetyltransferase/genetics, DNA, Viral, Genes, Reporter, Mice, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, RNA Splicing, Receptors, Retinoic Acid/metabolism, Receptors, Thyroid Hormone/metabolism, Retinoid X Receptors, Simian virus 40/genetics, Transcription Factors/metabolism, Transcription, Genetic, Triiodothyronine/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:27
Last modification date
25/09/2019 6:10
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