Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_B5478097CE31
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy.
Journal
Scientific reports
Author(s)
Kishazi E., Dor M., Eperon S., Oberic A., Turck N., Hamedani M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
17/07/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
10792
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The aim was to investigate the levels of cytokines and soluble IL-6R in the tears of patients with thyroid-associated orbitopathy (TAO) disease. Schirmer's test was adopted to collect tears from TAO patients (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) and healthy subjects (N = 18, 10 women, 45.4 years (±18.7)). Lacrimal cytokines and soluble IL-6R (sIL-6R) were measured using a 10-plex panel (Meso Scale Discovery Company) and Invitrogen Human sIL-6R Elisa kit, respectively. Tear levels of IL-10, IL-12p70, IL-13, IL-6 and TNF-α appeared significantly higher in TAO patients than in healthy subjects. Interestingly, IL-10, IL-12p70 and IL-8 levels increased in tears whatever the form of TAO whereas IL-13, IL-6 and TNF-α levels were significantly elevated in inflammatory TAO patients, meaning with a clinical score activity (CAS) ≥ 3, compared to controls. Furthermore, only 3 cytokines were strongly positively correlated with CAS (IL-13 Spearman coeff. r: 0.703, p = 0.0005; IL-6 r: 0.553, p = 0.011; IL-8 r: 0.618, p = 0.004, respectively). Finally, tobacco use disturbed the levels of several cytokines, especially in patient suffering of TAO. The differential profile of lacrimal cytokines could be useful for the diagnosis of TAO patients. Nevertheless, the tobacco use of these patients should be taken into account in the interpretation of the cytokine levels.
Pubmed
Web of science
Open Access
Yes
Create date
03/08/2018 16:14
Last modification date
20/08/2019 15:23
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