Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor.

Details

Serval ID
serval:BIB_B52975D0D68E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor.
Journal
Cell
Author(s)
Feinstein P., Bozza T., Rodriguez I., Vassalli A., Mombaerts P.
ISSN
0092-8674 (Print)
ISSN-L
0092-8674
Publication state
Published
Issued date
11/06/2004
Peer-reviewed
Oui
Volume
117
Number
6
Pages
833-846
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Odorant receptors (ORs) provide the core determinant of identity for axons of olfactory sensory neurons (OSNs) to coalesce into glomeruli in the olfactory bulb. Here, using gene targeting in mice, we examine how the OR protein determines axonal identity. An OR::GFP fusion protein is present in axons, consistent with a direct function of ORs in axon guidance. When the OR coding region is deleted, we observe OSNs that coexpress other ORs that function in odorant reception and axonal identity. It remains unclear if such coexpression is normally prevented by negative feedback on OR gene choice. A drastic reduction in OR protein level produces axonal coalescence into novel, remote glomeruli. By contrast, chimeric ORs and ORs with minor mutations perturb axon outgrowth. Strikingly, the beta2 adrenergic receptor can substitute for an OR in glomerular formation when expressed from an OR locus. Thus, ORs have not evolved a unique function in axon guidance.
Keywords
Alleles, Animals, Cell Communication/genetics, Cell Differentiation/genetics, Cilia/genetics, Cilia/metabolism, Gene Expression Regulation, Developmental/genetics, Gene Targeting, Green Fluorescent Proteins, Growth Cones/metabolism, Growth Cones/ultrastructure, Luminescent Proteins, Mice, Mice, Transgenic, Mutation/genetics, Olfactory Bulb/cytology, Olfactory Bulb/growth & development, Olfactory Bulb/metabolism, Olfactory Receptor Neurons/cytology, Olfactory Receptor Neurons/growth & development, Olfactory Receptor Neurons/metabolism, Open Reading Frames/genetics, Receptors, Adrenergic, beta-2/genetics, Receptors, Adrenergic, beta-2/physiology, Receptors, Odorant/genetics, Receptors, Odorant/physiology, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Synapses/metabolism, Synapses/ultrastructure
Pubmed
Web of science
Open Access
Yes
Create date
24/10/2018 9:22
Last modification date
25/05/2024 7:14
Usage data