Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast.

Details

Serval ID
serval:BIB_B4FB4CCBB9BE
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast.
Journal
Methods in Cell Biology
Author(s)
Ullal P., Bhatia P., Martin S.G.
ISSN
0091-679X (Print)
ISSN-L
0091-679X
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
137
Pages
341-353
Language
english
Abstract
Cytokinesis is an essential cellular event that completes the cell division cycle. It begins with the assembly of an actomyosin contractile ring that undergoes constriction concomitant with the septum formation to divide the cell in two. Placement of the septum at the right position is important to ensure fidelity of the division process. In fission yeast, the medially placed nucleus is a major spatial cue to position the site of division. In this chapter, we describe a simple synthetic biology-based approach to displace the nucleus and study the consequence on division site positioning. We also describe how to perform fluorescence recovery after photobleaching to follow the dynamics of cytokinetic proteins at defined time points by live-cell microscopy.

Keywords
Actin Cytoskeleton/ultrastructure, Actomyosin/genetics, Actomyosin/ultrastructure, Cell Nucleus/genetics, Cell Nucleus/ultrastructure, Cytokinesis/genetics, Fluorescence Recovery After Photobleaching/methods, Microscopy/methods, Schizosaccharomyces/genetics, Schizosaccharomyces/ultrastructure, Cell division, Cytokinesis, Division site placement, Fission yeast Schizosaccharomyces pombe, Fluorescence recovery after photobleaching, FRAP, Live-cell microscopy, Nuclear displacement, Synthetic biology
Pubmed
Web of science
Create date
17/01/2017 19:43
Last modification date
03/03/2018 20:43
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