Antibiotic-Induced Cell Chaining Triggers Pneumococcal Competence by Reshaping Quorum Sensing to Autocrine-Like Signaling.

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State: Public
Version: Final published version
Serval ID
serval:BIB_B4439E9EC71E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antibiotic-Induced Cell Chaining Triggers Pneumococcal Competence by Reshaping Quorum Sensing to Autocrine-Like Signaling.
Journal
Cell reports
Author(s)
Domenech A., Slager J., Veening J.W.
ISSN
2211-1247 (Electronic)
Publication state
Published
Issued date
27/11/2018
Peer-reviewed
Oui
Volume
25
Number
9
Pages
2390-2400.e3
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Streptococcus pneumoniae can acquire antibiotic resistance by activation of competence and subsequent DNA uptake. Here, we demonstrate that aztreonam (ATM) and clavulanic acid (CLA) promote competence. We show that both compounds induce cell chain formation by targeting the d,d-carboxypeptidase PBP3. In support of the hypothesis that chain formation promotes competence, we demonstrate that an autolysin mutant (ΔlytB) is hypercompetent. Since competence is initiated by the binding of a small extracellular peptide (CSP) to a membrane-anchored receptor (ComD), we wondered whether chain formation alters CSP diffusion kinetics. Indeed, ATM or CLA presence affects competence synchronization by shifting from global to local quorum sensing, as CSP is primarily retained to chained cells, rather than shared in a common pool. Importantly, autocrine-like signaling prolongs the time window in which the population is able to take up DNA. Together, these insights demonstrate the versatility of quorum sensing and highlight the importance of an accurate antibiotic prescription.
Keywords
CSP, Streptococcus pneumoniae, antibiotics, aztreonam, bacterial stress response, clavulanic acid, competence, contact-dependent signaling, quorum sensing, transformation
Pubmed
Web of science
Open Access
Yes
Create date
13/12/2018 13:09
Last modification date
20/08/2019 16:22
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