Protein kinase GCN2 mediates responses to glyphosate in Arabidopsis.

Details

Serval ID
serval:BIB_B439BAE215FA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Protein kinase GCN2 mediates responses to glyphosate in Arabidopsis.
Journal
BMC plant biology
Author(s)
Faus I., Zabalza A., Santiago J., Nebauer S.G., Royuela M., Serrano R., Gadea J.
ISSN
1471-2229 (Electronic)
ISSN-L
1471-2229
Publication state
Published
Issued date
21/01/2015
Peer-reviewed
Oui
Volume
15
Pages
14
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The increased selection pressure of the herbicide glyphosate has played a role in the evolution of glyphosate-resistance in weedy species, an issue that is becoming a threat to global agriculture. The molecular components involved in the cellular toxicity response to this herbicide at the expression level are still unidentified.
In this study, we identify the protein kinase GCN2 as a cellular component that fosters the action of glyphosate in the model plant Arabidopsis thaliana. Comparative studies using wild-type and gcn2 knock-out mutant seedlings show that the molecular programme that the plant deploys after the treatment with the herbicide, is compromised in gcn2. Moreover, gcn2 adult plants show a lower inhibition of photosynthesis, and both seedlings and adult gcn2 plants accumulate less shikimic acid than wild-type after treatment with glyphosate.
These results points to an unknown GCN2-dependent factor involved in the cascade of events triggered by glyphosate in plants. Data suggest either that the herbicide does not equally reach the target-enzyme in a gcn2 background, or that a decreased flux in the shikimate pathway in a gcn2 plants minimize the impact of enzyme inhibition.
Keywords
Arabidopsis/drug effects, Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Gene Expression Regulation, Plant/drug effects, Glycine/analogs & derivatives, Glycine/pharmacology, Mutation/genetics, Photosynthesis/drug effects, Photosystem II Protein Complex/metabolism, Plant Stomata/drug effects, Plant Stomata/physiology, Plant Transpiration/drug effects, Protein Kinases/genetics, Protein Kinases/metabolism, Seedlings/drug effects, Seedlings/genetics, Shikimic Acid/metabolism, Transcriptome/drug effects, Transcriptome/genetics
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2019 17:19
Last modification date
21/08/2019 5:35
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