IL-17F co-expression improves cell growth characteristics and enhances recombinant protein production during CHO cell line engineering.

Details

Serval ID
serval:BIB_B437F876A1C9
Type
Article: article from journal or magazin.
Collection
Publications
Title
IL-17F co-expression improves cell growth characteristics and enhances recombinant protein production during CHO cell line engineering.
Journal
Biotechnology and Bioengineering
Author(s)
Contie M., Leger O., Fouque N., Poitevin Y., Kosco-Vilbois M., Mermod N., Elson G.
ISSN
1097-0290 (Electronic)
ISSN-L
0006-3592
Publication state
Published
Issued date
2013
Volume
110
Number
4
Pages
1153-1163
Language
english
Abstract
The generation of a high productivity cell line is a critical step in the production of a therapeutic protein. Many innovative engineering strategies have been devised in order to maximize the expression rate of production cells for increased process efficiency. Less effort has focused on improvements to the cell line generation process, which is typically long and laborious when using mammalian cells. Based on unexpected findings when generating stable CHO cell lines expressing human IL-17F, we studied the benefit of expressing this protein during the establishment of production cell lines. We demonstrate that IL-17F expression enhances the rate of selection and overall number of selected cell lines as well as their transgene expression levels. We also show that this benefit is observed with different parental CHO cell lines and selection systems. Furthermore, IL-17F expression improves the efficiency of cell line subcloning processes. IL-17F can therefore be exploited in a standard manufacturing process to obtain higher productivity clones in a reduced time frame.
Keywords
Animals, CHO Cells, Cell Division, Cricetinae, Cricetulus, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Genetic Vectors, Humans, Interleukin-17/genetics, Recombinant Proteins/biosynthesis
Pubmed
Web of science
Create date
28/01/2014 13:28
Last modification date
20/08/2019 16:22
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