Dual effect of temperature on the human epithelial Na+ channel

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Serval ID
serval:BIB_B42A02E0F488
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dual effect of temperature on the human epithelial Na+ channel
Journal
Pflügers Archiv : European Journal of Physiology
Author(s)
Chraibi  A., Horisberger  J. D.
ISSN
0031-6768 (Print)
Publication state
Published
Issued date
12/2003
Volume
447
Number
3
Pages
316-320
Notes
Journal Article Research Support
Abstract
The amiloride-sensitive epithelial sodium channel (ENaC) is the rate-limiting step for sodium reabsorption in the distal segments of the nephron, in the colon and in the airways. Its activity is regulated by intracellular and extracellular factors but the mechanisms of this regulation are not yet completely understood. Recently, we have shown that the fast regulation of ENaC by the extracellular [Na+], a phenomenon termed self-inhibition, is temperature dependent. In the present study we examined the effects of temperature on the single-channel properties of ENaC. Single-channel recordings from excised patches showed that the channel open probability (Po, estimated from the number of open channels N.Po, where N is the total number of channels) increased on average two- to threefold while the single-channel conductance decreased by about half when the temperature of the perfusion solution was lowered from approximately 30 to approximately 15 degrees C. The effects of temperature on the single-channel conductance and Po explain the changes of the macroscopic current that can be observed upon temperature changes and, in particular, the paradoxical effect of temperature on the current carried by ENaC.
Keywords
Amiloride/pharmacology Animals Epithelial Cells/drug effects/*physiology Female Humans Ion Channel Gating/drug effects/physiology Oocytes/drug effects/physiology Sodium Channels/*physiology *Temperature Xenopus laevis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 12:38
Last modification date
01/10/2019 6:19
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