Surface markers of cloned human T cells with various cytolytic activities

Details

Serval ID
serval:BIB_B427481E9CEF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Surface markers of cloned human T cells with various cytolytic activities
Journal
Journal of Experimental Medicine
Author(s)
Moretta  L., Mingari  M. C., Sekaly  P. R., Moretta  A., Chapuis  B., Cerottini  J. C.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
08/1981
Volume
154
Number
2
Pages
569-74
Notes
Journal Article --- Old month value: Aug 1
Abstract
Human T cells stimulated in secondary allogeneic mixed lymphocyte culture (MLC) were cloned under limiting conditions in microculture systems using T cell growth factor and irradiated allogeneic cells. Clones with lytic activity against either phytohemagglutinin-induced blast cells bearing the stimulating alloantigen(s) (cytotoxic T lymphocyte [CTL] activity), L1210 mouse lymphoma cells coated with rabbit antibody (antibody-dependent cell-mediated cytotoxicity [ADCC]), or K562 human target cells were selected, expanded, and then analyzed for different surface markers, including rosette formation with sheep erythrocytes (E rosettes), receptors for the fc portion of IgG or IgM (Fc gamma R and Fc mu R), and a group of antigens recognized by monoclonal antibodies including Ia, 4F2, OKT8,a nd OKT4. All the cytotoxic cells were E rosette+, Ia+ and 4f2+. Expression of Fc gamma R was restricted to the clones active in ADCC. CTL clones were either OKT8+ or OKT8-. Furthermore, three of the OKT8- CTL clones were OKT4+. In addition, some cytolytic clones devoid of specific CTL activity were OKT8+. It thus appears that the claim that human CTL are OKT8+, OKT4-, and Ia- is not supported by the analysis of their phenotype at the clonal level.
Keywords
Antibodies/analysis Antibodies, Monoclonal Antibody-Dependent Cell Cytotoxicity Clone Cells/immunology Humans Killer Cells/immunology Lymphocyte Activation Receptors, Immunologic/analysis T-Lymphocytes/*classification
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:13
Last modification date
09/05/2019 0:02
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