Meta-analysis of heat- and chemically upregulated chaperone genes in plant and human cells.

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Serval ID
serval:BIB_B36E4A480341
Type
Article: article from journal or magazin.
Collection
Publications
Title
Meta-analysis of heat- and chemically upregulated chaperone genes in plant and human cells.
Journal
Cell Stress and Chaperones
Author(s)
Finka A., Mattoo R.U., Goloubinoff P.
ISSN
1466-1268 (Electronic)
ISSN-L
1355-8145
Publication state
Published
Issued date
2011
Volume
16
Number
1
Pages
15-31
Language
english
Abstract
Molecular chaperones are central to cellular protein homeostasis. In mammals, protein misfolding diseases and aging cause inflammation and progressive tissue loss, in correlation with the accumulation of toxic protein aggregates and the defective expression of chaperone genes. Bacteria and non-diseased, non-aged eukaryotic cells effectively respond to heat shock by inducing the accumulation of heat-shock proteins (HSPs), many of which molecular chaperones involved in protein homeostasis, in reducing stress damages and promoting cellular recovery and thermotolerance. We performed a meta-analysis of published microarray data and compared expression profiles of HSP genes from mammalian and plant cells in response to heat or isothermal treatments with drugs. The differences and overlaps between HSP and chaperone genes were analyzed, and expression patterns were clustered and organized in a network. HSPs and chaperones only partly overlapped. Heat-shock induced a subset of chaperones primarily targeted to the cytoplasm and organelles but not to the endoplasmic reticulum, which organized into a network with a central core of Hsp90s, Hsp70s, and sHSPs. Heat was best mimicked by isothermal treatments with Hsp90 inhibitors, whereas less toxic drugs, some of which non-steroidal anti-inflammatory drugs, weakly expressed different subsets of Hsp chaperones. This type of analysis may uncover new HSP-inducing drugs to improve protein homeostasis in misfolding and aging diseases.
Keywords
Anti-Inflammatory Agents, Non-Steroidal/pharmacology, Arabidopsis/genetics, Arabidopsis/metabolism, Cell Line, Computational Biology, Gene Regulatory Networks, Heat-Shock Proteins/genetics, Heat-Shock Proteins/metabolism, Hot Temperature, Humans, Molecular Chaperones/genetics, Molecular Chaperones/metabolism, Monocytes/metabolism, Unfolded Protein Response/genetics, Up-Regulation
Pubmed
Web of science
Open Access
Yes
Create date
18/05/2011 10:28
Last modification date
01/10/2019 7:19
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