Na+,K(+)-ATPase activity and hormones in single nephron segments from nephrotic rats.

Details

Serval ID
serval:BIB_B34F5E241637
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Na+,K(+)-ATPase activity and hormones in single nephron segments from nephrotic rats.
Journal
Clinical Science
Author(s)
Vogt B., Favre H.
ISSN
0143-5221
Publication state
Published
Issued date
06/1991
Peer-reviewed
Oui
Volume
80
Number
6
Pages
599-604
Language
english
Abstract
1. In the nephrotic syndrome the kidneys retain salt and water, which leads to oedema formation. The site of this sodium retention has been localized in the cortical collecting tubule by micropuncture studies. Whether or not this phenomenon is an intrinsic renal problem or is the consequence of changes in hormonal activities is still a matter of discussion. 2. Using the model of puromycin aminonucleoside-induced nephrotic syndrome in the rat, we measured Na+,K(+)-ATPase activity in nephron segments from control and nephrotic rats and investigated the regulatory role of aldosterone and endogenous-ouabain-displacing factor. 3. Nephrotic animals had a twofold increase in Na+,K(+)-ATPase activity in the cortical collecting tubule only (control versus nephrotic: 1065 +/- 68 versus 2081 +/- 274 pmol h-1 mm-1, P = 0.036), which was not modified by adrenalectomy and was independent of the kidney content of endogenous ouabain-displacing factor. Na+,K(+)-ATPase activity in the cortical collecting tubule correlated with the sodium balance in both control and nephrotic rats. 4. The data are consistent with the view that sodium retention in this model of the nephrotic syndrome is a primary event, i.e. an increase in sodium transport throughout the cortical collecting tubule expressed as a twofold increase in Na+,K(+)-ATPase activity which is no longer under hormonal regulation (aldosterone and endogenous ouabain-displacing factor).
Keywords
Adrenalectomy, Aldosterone/physiology, Animals, Cardenolides, Digoxin, Kidney/chemistry, Male, Nephrons/metabolism, Nephrotic Syndrome/metabolism, Proteins/metabolism, Puromycin Aminonucleoside, Rats, Rats, Inbred Strains, Saponins, Sodium/metabolism, Sodium-Potassium-Exchanging ATPase/analysis, Sodium-Potassium-Exchanging ATPase/antagonists &amp, inhibitors
Pubmed
Web of science
Create date
25/01/2008 13:03
Last modification date
20/08/2019 15:21
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