Magnitude of CD8 T-cell responses against hepatitis C virus and severity of hepatitis do not necessarily determine outcomes in acute hepatitis C virus infection.

Details

Serval ID
serval:BIB_B2EC97853AB8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Magnitude of CD8 T-cell responses against hepatitis C virus and severity of hepatitis do not necessarily determine outcomes in acute hepatitis C virus infection.
Journal
Hepatology Research
Author(s)
Doi H., Hiroishi K., Shimazaki T., Eguchi J., Baba T., Ito T., Matsumura T., Nozawa H., Morikawa K., Ishii S., Hiraide A., Sakaki M., Imawari M.
ISSN
1386-6346
Publication state
Published
Issued date
2009
Volume
39
Number
3
Pages
256-265
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Aim: We investigated the relationship between the magnitude of comprehensive hepatitis C virus (HCV)-specific CD8(+) T-cell responses and the clinical course of acute HCV infection. Methods: Six consecutive patients with acute HCV infection were studied. Analysis of HCV-specific CD8(+) T-cell responses was performed using an interferon-gamma-based enzyme-linked immunospot assay using peripheral CD8(+) T-cells, monocytes and 297 20-mer synthetic peptides overlapping by 10 residues and spanning the entire HCV sequence of genotype 1b. Results: Five patients presented detectable HCV-specific CD8(+) T-cell responses against a single and different peptide, whereas 1 patient showed responses against three different peptides. Neither the magnitude of HCV-specific CD8(+) T-cell responses nor the severity of hepatitis predicts the outcome of acute hepatitis. The maximum number of HCV-specific CD8(+) T-cells correlated with maximum serum alanine aminotransferase level during the course (r = 0.841, P = 0.036). Conclusions: HCV-specific CD8(+) T-cell responses were detectable in all 6 patients with acute HCV infection, and 6 novel HCV-specific CTL epitopes were identified. Acute HCV infection can resolve with detectable HCV-specific CD8(+) T-cell responses, but without development of antibody against HCV.
Pubmed
Web of science
Create date
21/01/2010 17:29
Last modification date
20/08/2019 15:21
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