A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
Details
Serval ID
serval:BIB_B22C1FCEE001
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A role for Drosophila LKB1 in anterior-posterior axis formation and epithelial polarity.
Journal
Nature
ISSN
0028-0836 (Print)
ISSN-L
0028-0836
Publication state
Published
Issued date
2003
Volume
421
Number
6921
Pages
379-384
Language
english
Abstract
The PAR-4 and PAR-1 kinases are necessary for the formation of the anterior-posterior (A-P) axis in Caenorhabditis elegans. PAR-1 is also required for A-P axis determination in Drosophila. Here we show that the Drosophila par-4 homologue, lkb1, is required for the early A-P polarity of the oocyte, and for the repolarization of the oocyte cytoskeleton that defines the embryonic A-P axis. LKB1 is phosphorylated by PAR-1 in vitro, and overexpression of LKB1 partially rescues the par-1 phenotype. These two kinases therefore function in a conserved pathway for axis formation in flies and worms. lkb1 mutant clones also disrupt apical-basal epithelial polarity, suggesting a general role in cell polarization. The human homologue, LKB1, is mutated in Peutz-Jeghers syndrome and is regulated by prenylation and by phosphorylation by protein kinase A. We show that protein kinase A phosphorylates Drosophila LKB1 on a conserved site that is important for its activity. Thus, Drosophila and human LKB1 may be functional homologues, suggesting that loss of cell polarity may contribute to tumour formation in individuals with Peutz-Jeghers syndrome.
Keywords
Amino Acid Sequence, Animals, Body Patterning, Cell Polarity, Congo Red, Conserved Sequence, Cyclic AMP-Dependent Protein Kinases/metabolism, Drosophila Proteins/chemistry, Drosophila Proteins/genetics, Drosophila melanogaster/cytology, Drosophila melanogaster/embryology, Epithelial Cells/cytology, Genes, Insect/genetics, Humans, Molecular Sequence Data, Peutz-Jeghers Syndrome/enzymology, Phosphorylation, Protein Transport, Protein-Serine-Threonine Kinases/chemistry, Protein-Serine-Threonine Kinases/genetics, RNA, Messenger/genetics, RNA, Messenger/metabolism
Pubmed
Web of science
Create date
18/03/2008 11:32
Last modification date
20/08/2019 15:20