A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype.

Details

Serval ID
serval:BIB_B20F6CE182AD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype.
Journal
Stem Cells
Author(s)
Majd H., Wipff P.J., Buscemi L., Bueno M., Vonwil D., Quinn T.M., Hinz B.
ISSN
1549-4918 (Electronic)
ISSN-L
1066-5099
Publication state
Published
Issued date
2009
Volume
27
Number
1
Pages
200-209
Language
english
Abstract
Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferation. This approach maintains constantly high cell density while preventing contact inhibition of growth. A highly elastic culture surface was enlarged in steps of 5% over the course of a 20-day culture period to 800% of the initial surface area. Nine weeks of dynamic expansion culture produced 10-fold more MSC compared with conventional culture, with one-third the number of trypsin passages. After 9 weeks, MSC continued to proliferate under dynamic expansion but ceased to grow in conventional culture. Dynamic expansion culture fully retained the multipotent character of MSC, which could be induced to differentiate into adipogenic, chondrogenic, osteogenic, and myogenic lineages. Development of an undesired fibrogenic myofibroblast phenotype was suppressed. Hence, our novel method can rapidly provide the high number of autologous, multipotent, and nonfibrogenic MSC needed for successful regenerative medicine.
Keywords
Cell Culture Techniques/methods, Cell Differentiation, Cell Lineage, Cell Proliferation, Humans, Mesenchymal Stromal Cells/cytology, Phenotype, Silicone Elastomers
Pubmed
Web of science
Open Access
Yes
Create date
13/02/2014 15:40
Last modification date
20/08/2019 15:20
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