Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures.

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Version: Final published version
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Serval ID
serval:BIB_B1E7CA94FF35
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures.
Journal
The Journal of experimental medicine
Author(s)
Cerottini J.C., Engers H.D., Macdonald H.R., Brunner T.
ISSN
0022-1007
ISSN-L
0022-1007
Publication state
Published
Issued date
01/09/1974
Peer-reviewed
Oui
Volume
140
Number
3
Pages
703-717
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Mouse cytotoxic T lymphocytes (CTL) were generated in mixed leukocyte cultures (MLC) using spleen cells as responding cells and irradiated allogeneic spleen cells as stimulating cells. Cytotoxicity was assessed by a quantitative (51)Cr assay system and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Inclusion of 2-mercaptoethanol in the MLC medium resulted in a 20-40-fold increase in the relative number of CTL generated at the peak of the response. Under these culture conditions, cell-mediated cytotoxic activity was detectable in MLC populations as early as 48 h after the onset of the cultures. When spleen cells from mice immunized with allogeneic tumor cells 2-4 mo previously were cultured with irradiated spleen cells of the same alloantigenic specificity (MLC-Imm), it was found that the cell-mediated cytotoxic response was detectable earlier and reached higher levels than that observed in a primary MLC. At the peak of the response, MLC-Imm populations were observed to lyse up to 50% of the target cells within 3 h at a lymphocyte: target cell ratio of 0.3:1. Immunological and physical characterization of the effector cells generated in MLC-Imm indicated that they were medium to large-sized T lymphocytes. Altogether, these studies suggested the existence of an anamnestic cell-mediated cytotoxic response in MLC-Imm.
Keywords
Animals, Antigen-Antibody Reactions/drug effects, Antigen-Antibody Reactions/radiation effects, Chromium Radioisotopes, Cytotoxicity Tests, Immunologic, Immunity, Cellular, Isoantigens, Kinetics, Lymphocyte Culture Test, Mixed, Mercaptoethanol/pharmacology, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred DBA, Spleen/cytology, Spleen/immunology, T-Lymphocytes/drug effects, T-Lymphocytes/immunology, T-Lymphocytes/radiation effects
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:13
Last modification date
09/08/2024 15:51
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