Triiodothyronine has diverse and multiple stimulating effects on expression of the major myelin protein genes.

Details

Serval ID
serval:BIB_B19BA6994386
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Triiodothyronine has diverse and multiple stimulating effects on expression of the major myelin protein genes.
Journal
Journal of Neurochemistry
Author(s)
Tosic M., Torch S., Comte V., Dolivo M., Honegger P., Matthieu J.M.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Publication state
Published
Issued date
1992
Volume
59
Number
5
Pages
1770-1777
Language
english
Abstract
If the importance of triiodothyronine (T3) on brain development including myelinogenesis has long been recognized, its mechanism of action at the gene level is still not fully elucidated. We studied the effect of T3 on the expression of myelin protein genes in aggregating brain cell cultures. T3 increases the concentrations of mRNA transcribed from the following four myelin protein genes: myelin basic protein (Mbp), myelin-associated glycoprotein (Mag), proteolipid protein (Plp), and 2',3'-cyclic nucleotide 3'-phosphodiesterase (Cnp). T3 is not only a triggering signal for oligodendrocyte differentiation, but it has continuous stimulatory effects on myelin gene expression. Transcription in isolated nuclei experiments shows that T3 increases Mag and Cnp transcription rates. After inhibiting transcription with actinomycin D, we measured the half-lives of specific mRNAs. Our results show that T3 increases the stability of mRNA for myelin basic protein, and probably proteolipid protein. In vitro translation followed by myelin basic protein-specific immunoprecipitation showed a direct stimulatory effect of T3 on myelin basic protein mRNA translation. Moreover, this stimulation was higher when the mRNA was already stabilized in culture, indicating that stabilization is achieved through mRNA structural modifications. These results demonstrate the diverse and multiple mechanisms of T3 stimulation of myelin protein genes.
Keywords
Animals, Cells, Cultured, Gene Expression Regulation/drug effects, Myelin Basic Protein/biosynthesis, Myelin Basic Protein/drug effects, Protein Biosynthesis/drug effects, RNA, Messenger/drug effects, Rats, Telencephalon/drug effects, Telencephalon/metabolism, Transcription, Genetic/drug effects, Triiodothyronine/pharmacology
Pubmed
Web of science
Create date
24/01/2008 13:12
Last modification date
20/08/2019 15:20
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