Developing yeast models of human neurodegenerative disorders.

Details

Serval ID
serval:BIB_B10EC75E080F
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Developing yeast models of human neurodegenerative disorders.
Journal
Methods in molecular biology
Author(s)
Ocampo A., Barrientos A.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
793
Pages
113-127
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Neurodegenerative diseases represent one of the most devastating types of diseases in older populations in our time. Significant efforts have been made over the last 20 years to understand the molecular, biochemical, and physiological alterations underlying these diseases. However, in most cases, little is known about their pathological mechanisms due to their high complexity and involvement of a multiplicity of cellular pathways. To gain insight into this group of disorders and to devise potential therapeutic approaches, cellular and animal models of neurodegenerative proteinopathies have been created. Among them, the yeast Saccharomyces cerevisiae has been one of the most popular model organisms due to the degree of conservation of many biological pathways from yeast to human as well as its ease of use. Here, we describe how to create yeast models of neurodegenerative proteinopathies by ectopic expression of human proteins and how to perform a basic characterization of these models by analyzing cellular toxicity and protein aggregation.
Keywords
Blotting, Western, Cell Line, Fluorescent Dyes/metabolism, Humans, Microscopy, Fluorescence, Models, Biological, Neurodegenerative Diseases/genetics, Neurodegenerative Diseases/metabolism, Protein Multimerization, Protein Structure, Quaternary, Recombinant Fusion Proteins/chemistry, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Transformation, Genetic
Pubmed
Create date
14/08/2018 9:49
Last modification date
20/08/2019 15:20
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