Localization and production of plasminogen activator inhibitor-1 in human healthy and atherosclerotic arteries.

Details

Serval ID
serval:BIB_B0942F5C3920
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Localization and production of plasminogen activator inhibitor-1 in human healthy and atherosclerotic arteries.
Journal
Arteriosclerosis and thrombosis
Author(s)
Lupu F., Bergonzelli G.E., Heim D.A., Cousin E., Genton C.Y., Bachmann F., Kruithof E.K.
ISSN
1049-8834 (Print)
ISSN-L
1049-8834
Publication state
Published
Issued date
07/1993
Peer-reviewed
Oui
Volume
13
Number
7
Pages
1090-1100
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
High plasma levels of plasminogen activator inhibitor type-1 (PAI-1), the principal inhibitor of the fibrinolytic system, have been associated with thrombotic and arterial disease. To study PAI-1 expression in healthy and atherosclerotic human arteries, a detailed analysis was made by light and electron microscopy immunocytochemistry and by in situ hybridization. In healthy arteries PAI-1 was found both at the level of endothelial cells and of smooth muscle cells (SMCs) of the arterial media. In early atherosclerotic lesions PAI-1 was also detected in intimal SMCs and in extracellular areas in association with vitronectin. Immunogold analysis by electron microscopy revealed PAI-1 in vesicular structures in endothelial cells and in SMCs with normal or foam cell characteristics. In advanced atheromatous plaques, PAI-1 mRNA expression in SMCs within the fibrous cap was increased compared with SMCs located in the adjacent media or in normal arterial tissue. PAI-1 mRNA was also detected in macrophages located at the periphery of the necrotic core. The increased synthesis of PAI-1 by cellular components of the atherosclerotic plaque and the extracellular accumulation of PAI-1 may contribute to the thrombotic complications associated with plaque rupture and possibly play a role in the accumulation of extracellular matrix deposits.
Keywords
Arteries/chemistry, Arteries/metabolism, Arteries/ultrastructure, Arteriosclerosis/metabolism, Blood Proteins/analysis, Endothelium, Vascular/cytology, Endothelium, Vascular/metabolism, Endothelium, Vascular/ultrastructure, Glycoproteins/analysis, Humans, Immunohistochemistry, In Situ Hybridization, Microscopy, Electron, Muscle, Smooth, Vascular/cytology, Muscle, Smooth, Vascular/metabolism, Plasminogen Activator Inhibitor 1/analysis, Plasminogen Activator Inhibitor 1/biosynthesis, Subcellular Fractions/chemistry, Vitronectin
Pubmed
Web of science
Open Access
Yes
Create date
18/05/2014 16:48
Last modification date
25/05/2024 7:12
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