Meta-omics-aided isolation of an elusive anaerobic arsenic-methylating soil bacterium.

Details

Serval ID
serval:BIB_B03B7173E581
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Meta-omics-aided isolation of an elusive anaerobic arsenic-methylating soil bacterium.
Journal
The ISME journal
Author(s)
Viacava K., Qiao J., Janowczyk A., Poudel S., Jacquemin N., Meibom K.L., Shrestha H.K., Reid M.C., Hettich R.L., Bernier-Latmani R.
ISSN
1751-7370 (Electronic)
ISSN-L
1751-7362
Publication state
Published
Issued date
07/2022
Peer-reviewed
Oui
Volume
16
Number
7
Pages
1740-1749
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Soil microbiomes harbour unparalleled functional and phylogenetic diversity. However, extracting isolates with a targeted function from complex microbiomes is not straightforward, particularly if the associated phenotype does not lend itself to high-throughput screening. Here, we tackle the methylation of arsenic (As) in anoxic soils. As methylation was proposed to be catalysed by sulfate-reducing bacteria. However, to date, there are no available anaerobic isolates capable of As methylation, whether sulfate-reducing or otherwise. The isolation of such a microorganism has been thwarted by the fact that the anaerobic bacteria harbouring a functional arsenite S-adenosylmethionine methyltransferase (ArsM) tested to date did not methylate As in pure culture. Additionally, fortuitous As methylation can result from the release of non-specific methyltransferases upon lysis. Thus, we combined metagenomics, metatranscriptomics, and metaproteomics to identify the microorganisms actively methylating As in anoxic soil-derived microbial cultures. Based on the metagenome-assembled genomes of microorganisms expressing ArsM, we isolated Paraclostridium sp. strain EML, which was confirmed to actively methylate As anaerobically. This work is an example of the application of meta-omics to the isolation of elusive microorganisms.
Keywords
Anaerobiosis, Arsenic, Bacteria, Anaerobic/genetics, Phylogeny, Soil, Sulfates
Pubmed
Web of science
Open Access
Yes
Create date
09/04/2022 19:40
Last modification date
15/07/2022 6:35
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