Actions of VIP, hGRF, PHI and secretin: comparative studies in cerebral cortex and adenohypophysis
Details
Serval ID
serval:BIB_AF4A303D0164
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Actions of VIP, hGRF, PHI and secretin: comparative studies in cerebral cortex and adenohypophysis
Journal
Peptides
ISSN
0196-9781
Publication state
Published
Issued date
1986
Peer-reviewed
Oui
Volume
7 Suppl 1
Pages
175-80
Notes
Comparative Study
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Abstract
We have examined the effects of hGRF on cyclic AMP and glycogen levels in mouse cerebral cortical slices. hGRF-44-NH2 and hGRF-28-OH did not stimulate cyclic AMP formation nor glycogenolysis and did not antagonize the stimulatory effects of VIP on cyclic AMP formation and glycogenolysis. These observations indicate that despite the structural homologies with VIP, hGRF does not interact with VIP receptors coupled to adenylate cyclase in mouse cerebral cortex. This is in contrast with observations in other tissues and species, such as rat and human intestinal epithelial membranes and rat pancreas. We have also compared the effects of hGRF, VIP, PHI and secretin on Growth Hormone (GH) release and cyclic AMP levels in anterior pituitary cells in vitro. VIP and PHI, but not secretin, promote at a high concentration (10(-6) M) a small but significant release of GH. This GH release is accompanied by increases in cyclic AMP levels. The concentration of VIP and PHI required to elicit these effects is high and suggests that VIP and PHI act as low affinity pharmacological analogs of hGRF on hGRF pituitary receptors.
Keywords
Animals
Cerebral Cortex/drug effects/physiology
Cyclic AMP/metabolism
Glycogen/metabolism
Growth Hormone/secretion
Growth Hormone-Releasing Hormone/*pharmacology
Male
Mice
Peptide PHI
Peptides/*pharmacology
Pituitary Gland, Anterior/drug effects/physiology
Secretin/*pharmacology
Vasoactive Intestinal Peptide/metabolism/*pharmacology
Pubmed
Web of science
Create date
20/01/2008 18:22
Last modification date
20/08/2019 15:18