Minority potassium-uptake system Trk2 has a crucial role in yeast survival of glucose-induced cell death.

Details

Serval ID
serval:BIB_AF49DF4778CC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Minority potassium-uptake system Trk2 has a crucial role in yeast survival of glucose-induced cell death.
Journal
Microbiology
Author(s)
Dušková M., Cmunt D., Papoušková K., Masaryk J., Sychrová H.
ISSN
1465-2080 (Electronic)
ISSN-L
1350-0872
Publication state
Published
Issued date
06/2021
Peer-reviewed
Oui
Volume
167
Number
6
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The existence of programmed cell death in Saccharomyces cerevisiae has been reported for many years. Glucose induces the death of S. cerevisiae in the absence of additional nutrients within a few hours, and the absence of active potassium uptake makes cells highly sensitive to this process. S. cerevisiae cells possess two transporters, Trk1 and Trk2, which ensure a high intracellular concentration of potassium, necessary for many physiological processes. Trk1 is the major system responsible for potassium acquisition in growing and dividing cells. The contribution of Trk2 to potassium uptake in growing cells is almost negligible, but Trk2 becomes crucial for stationary cells for their survival of some stresses, e.g. anhydrobiosis. As a new finding, we show that both Trk systems contribute to the relative thermotolerance of S. cerevisiae BY4741. Our results also demonstrate that Trk2 is much more important for the cell survival of glucose-induced cell death than Trk1, and that stationary cells deficient in active potassium uptake lose their ATP stocks more rapidly than cells with functional Trk systems. This is probably due to the upregulated activity of plasma-membrane Pma1 H <sup>+</sup> -ATPase, and consequently, it is the reason why these cells die earlier than cells with functional active potassium uptake.
Keywords
Cation Transport Proteins/genetics, Cation Transport Proteins/metabolism, Cell Death, Glucose/metabolism, Microbial Viability, Potassium/metabolism, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/growth & development, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, ATP content, GICD, Saccharomyces cerevisiae, potassium uptake, stationary cells, thermotolerance
Pubmed
Create date
06/07/2021 12:43
Last modification date
04/01/2022 16:53
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