QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation Into Liposomes.

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State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_AF425E56083A
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation Into Liposomes.
Journal
Methods in molecular biology
Author(s)
Brunner L., Barnier-Quer C., Collin N.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
1494
Pages
73-86
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
QS-21, a saponin extracted from the tree Quillaja saponaria Molina, is a vaccine adjuvant which has been shown to elicit robust antibody and cell-mediated immune responses in a variety of preclinical and clinical studies [1]. Its purification from the natural source is a lengthy and difficult process. The commercially available saponin mixture Quil-A® is a fraction of the bark extract containing a variety of saponins, including QS-21. In order to facilitate access to QS-21 at laboratory-scale amounts, we propose here a method of purification of QS-21 starting from Quil-A®. In addition, we describe a protocol to appropriately formulate QS-21 into cholesterol-containing, neutral liposomes which are known to decrease QS-21's hemolytic activity while retaining the adjuvant effect. Methods for the physicochemical characterization of purified QS-21 and of the QS-21/liposome formulations are also described.

Keywords
Adjuvants, Immunologic/chemical synthesis, Adjuvants, Immunologic/chemistry, Adjuvants, Immunologic/isolation & purification, Adjuvants, Immunologic/pharmacology, Animals, Liposomes, Mice, Saponins/chemical synthesis, Saponins/chemistry, Saponins/isolation & purification, Saponins/pharmacology, QS-21, Saponin, Vaccine adjuvant
Pubmed
Open Access
Yes
Create date
19/10/2016 12:27
Last modification date
20/08/2019 15:18
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