Macrophage precursor cells produce perforin and perform Yac-1 lytic activity in response to stimulation with interleukin-2

Details

Serval ID
serval:BIB_AED16CECBE27
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Macrophage precursor cells produce perforin and perform Yac-1 lytic activity in response to stimulation with interleukin-2
Journal
Journal of Leukocyte Biology
Author(s)
Li  H., Pohler  U., Strehlow  I., Hertig  S., Baccarini  M., Emmendorffer  A., Tschopp  J., Lohmann-Matthes  M. L.
ISSN
0741-5400 (Print)
Publication state
Published
Issued date
08/1994
Volume
56
Number
2
Pages
117-23
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Abstract
Macrophage precursor cells, derived from mouse bone marrow culture with granulocyte-macrophage colony-stimulating factor or colony-stimulating factor 1 (CSF-1) as growth factor and interleukin-2 (IL-2) as stimulating factor, were activated by IL-2 to exert strong cytolytic activity against Yac-1 cells. In response to IL-2 stimulation these bone marrow macrophage precursor cells produced perforin as lytic molecules. The purity of the precursor cells for the study was proved as homogeneous positivity for Mac-1, NK-1.1 and negativity for Lyt 1 and 2. The cells express CSF-1 receptors on their surface, are able to proliferate and differentiate into typical macrophages when stimulated with CSF-1, and are therefore members of the macrophage lineage. Perforin transcripts were identified by Northern blot analysis of IL-2-treated macrophage precursor cells, and the presence of perforin protein in the cytoplasmic granules was demonstrated by immunohistochemical staining using a monoclonal antiperforin antibody. In addition, the biological activity of the perforin contained in the macrophage precursor's granules could be documented as calcium-dependent lytic activity using Yac-1 and sheep red blood cells as targets. The results presented in this paper imply the existence of a bipotent precursor cell, which can mature into a typical macrophage if CSF-1 or phorbol 12-myristate 13-acetate is supplied as differentiation stimulating factor but develops into an NK/LAK cell when early activation with IL-2 is provided.
Keywords
Animals Antigens/analysis Blotting, Northern Cytotoxicity, Immunologic DNA, Complementary/genetics Hematopoietic Stem Cells/*drug effects/*physiology Interleukin-2/*pharmacology Killer Cells, Natural/immunology/physiology Lymphoma/pathology Macrophages/*drug effects/*physiology Membrane Glycoproteins/*biosynthesis/genetics Mice Mice, Inbred C57BL Pore Forming Cytotoxic Proteins RNA, Messenger/biosynthesis/genetics Receptors, Colony-Stimulating Factor/genetics Stimulation, Chemical Tumor Cells, Cultured
Pubmed
Web of science
Create date
24/01/2008 15:19
Last modification date
20/08/2019 15:18
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