Epstein-Barr virus latency in kidney specimens from transplant recipients.

Details

Serval ID
serval:BIB_AE5CB4BC9AFE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Epstein-Barr virus latency in kidney specimens from transplant recipients.
Journal
Nephrology, dialysis, transplantation
Author(s)
Arias L.F., Hernández S., Prats D., Sanchez-Fructoso A., Márques M., Alvarez T., Barrientos A., Blanco J.
ISSN
0931-0509 (Print)
ISSN-L
0931-0509
Publication state
Published
Issued date
12/2003
Peer-reviewed
Oui
Volume
18
Number
12
Pages
2638-2643
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Epstein-Barr virus (EBV) infection is common in immunosuppressed patients and can lead to life threatening lymphoproliferative diseases. Small numbers of cells infected by EBV have been detected in human tissues, transplanted or non-transplanted. Little is known about EBV latency in the allograft kidneys of patients without post-transplant lymphoproliferative disease (PTLD). The aims of this study were to look for the presence of EBV-encoded small RNAs (EBER) in allograft kidneys and to quantify their expression.
We analysed 62 allograft nephrectomies and 20 native kidneys to determine the presence of EBV; we also quantified its expression and calculated its ratios to CD45 and CD20 cells. The techniques used were: tissue microarray, EBER-1- and 2-specific in situ hybridization and immunohistochemistry.
EBER expression was detected in 30.6% of transplanted kidneys and 5% of non-transplanted kidneys. In the positive specimens, a mean of 8.2 cells/1.57 mm(2) expressed the EBERs (range 1-38 cells). The ratios of EBER-positive (+) cells to CD45 or CD20 cells were 1.7 +/- 2.4% (range 0.1-8.1%) and 8.4 +/- 10.9% (range 0.5-34.4%), respectively. No relationship was found between anti-T-cell treatment and EBER expression in the failed allografts.
In failed kidney allografts, a small number of lymphocytes can express EBV latency. The number of EBER+ cells is smaller than in PTLD. Studies of functioning grafts are necessary to better understand the clinical relevance of this expression.

Keywords
Adult, Aged, Epstein-Barr Virus Infections/virology, Female, Herpesvirus 4, Human/isolation & purification, Humans, Kidney Transplantation/immunology, Male, Middle Aged, RNA, Viral/isolation & purification, Transplants/virology
Pubmed
Web of science
Create date
13/02/2018 10:36
Last modification date
20/08/2019 16:18
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