Mapping the transcription start points of the Staphylococcus aureus eap, emp, and vwb promoters reveals a conserved octanucleotide sequence that is essential for expression of these genes.

Details

Serval ID
serval:BIB_AD9B717B855D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mapping the transcription start points of the Staphylococcus aureus eap, emp, and vwb promoters reveals a conserved octanucleotide sequence that is essential for expression of these genes.
Journal
Journal of Bacteriology
Author(s)
Harraghy N., Homerova D., Herrmann M., Kormanec J.
ISSN
1098-5530[electronic], 0021-9193[linking]
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
190
Number
1
Pages
447-451
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Mapping the transcription start points of the eap, emp, and vwb promoters revealed a conserved octanucleotide sequence (COS). Deleting this sequence abolished the expression of eap, emp, and vwb. However, electrophoretic mobility shift assays gave no evidence that this sequence was a binding site for SarA or SaeR, known regulators of eap and emp.
Keywords
Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, Base Sequence, Binding Sites, Conserved Sequence, Extracellular Matrix Proteins/chemistry, Extracellular Matrix Proteins/metabolism, Gene Expression Regulation, Bacterial, Oligodeoxyribonucleotides/chemistry, Promoter Regions, Genetic, Staphylococcus aureus/genetics, Trans-Activators/chemistry, Trans-Activators/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
16/06/2010 12:25
Last modification date
20/08/2019 16:17
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