An analog to digital converter controls bistable transfer competence development of a widespread bacterial integrative and conjugative element.

Details

Serval ID
serval:BIB_AD5E010A817F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An analog to digital converter controls bistable transfer competence development of a widespread bacterial integrative and conjugative element.
Journal
eLife
Author(s)
Carraro N., Richard X., Sulser S., Delavat F., Mazza C., van der Meer J.R.
ISSN
2050-084X (Electronic)
ISSN-L
2050-084X
Publication state
Published
Issued date
28/07/2020
Peer-reviewed
Oui
Volume
9
Pages
e57915
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Conjugative transfer of the integrative and conjugative element ICEclc in Pseudomonas requires development of a transfer competence state in stationary phase, which arises only in 3-5% of individual cells. The mechanisms controlling this bistable switch between non-active and transfer competent cells have long remained enigmatic. Using a variety of genetic tools and epistasis experiments in P. putida, we uncovered an 'upstream' cascade of three consecutive transcription factor-nodes, which controls transfer competence initiation. One of the uncovered transcription factors (named BisR) is representative for a new regulator family. Initiation activates a feedback loop, controlled by a second hitherto unrecognized heteromeric transcription factor named BisDC. Stochastic modelling and experimental data demonstrated the feedback loop to act as a scalable converter of unimodal (population-wide or 'analog') input to bistable (subpopulation-specific or 'digital') output. The feedback loop further enables prolonged production of BisDC, which ensures expression of the 'downstream' functions mediating ICE transfer competence in activated cells. Phylogenetic analyses showed that the ICEclc regulatory constellation with BisR and BisDC is widespread among Gamma- and Beta-proteobacteria, including various pathogenic strains, highlighting its evolutionary conservation and prime importance to control the behaviour of this wide family of conjugative elements.
Keywords
Bacteria/genetics, Bacterial Outer Membrane Proteins/genetics, Bacterial Outer Membrane Proteins/metabolism, Conjugation, Genetic/physiology, DNA Transposable Elements, Gene Expression Regulation, Bacterial, Gene Transfer, Horizontal, Genome, Bacterial, Pseudomonas/genetics, Pseudomonas/metabolism, Transcription Factors/metabolism, Pseudomonas putida, clc element, conjugation, forward feedback loop, genetics, genomics, horizontal gene transfer, infectious disease, microbiology, transfer competence
Pubmed
Web of science
Open Access
Yes
Create date
11/08/2020 10:46
Last modification date
23/02/2021 7:26
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