Characterization of a protease of a feather-degrading Microbacterium species

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Serval ID
serval:BIB_AD4107E86DC6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Characterization of a protease of a feather-degrading Microbacterium species
Journal
Letters in Applied Microbiology
Author(s)
Thys  R.C.S., Lucas  F.S., Riffel  A., Heeb  P., Brandelli  A.
Publication state
Published
Issued date
2004
Volume
39
Pages
181-186
Notes
oums
Abstract
Methods and Results: The strain kr10 producing a high keratinolytic activity when cultured on native feather broth was identified as Microbacterium sp., based on phenotypical characteristics and 16S rDNA sequence. The bacterium presented optimum growth and feather-degrad ing activity at pH 7.0 and 30degreesC. Complete feather degradation was achieved during cultivation. The keratinase was partially purified by gel filtration chromatography. It was optimally active at pH 7.0 and 55degreesC. The enzyme was inhibited by 1,10-phenanthroline, EDTA, p-chloromercuribenzoic acid, 2-mercaptoethanol and metal ions like Hg2+, Cu2+ and Zn2+. Significance and Impact of the Study: A new Microbacterium sp. strain was characterized presenting high feather-degrading activity, which appears to be associated to a metalloprotease-type keratinase. This micro-organism has enormous potential for use in biotechnological processes involving keratin hydrolysis.
Create date
19/11/2007 11:44
Last modification date
20/08/2019 16:17
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