Activation of MAP kinase (ERK1/2) in human neonatal colonic enteric nervous system.

Details

Serval ID
serval:BIB_ACD1694F00DF
Type
Article: article from journal or magazin.
Collection
Publications
Title
Activation of MAP kinase (ERK1/2) in human neonatal colonic enteric nervous system.
Journal
Neurogastroenterology and Motility
Author(s)
Rouleau C., Matécki S., Kalfa N., Costes V., de Santa Barbara P.
ISSN
1365-2982 (Electronic)
ISSN-L
1350-1925
Publication state
Published
Issued date
2009
Volume
21
Number
2
Pages
207-214
Language
english
Abstract
The aim of this study was to examine mitogen-activated protein kinase (ERK1/2) activation in the human neonatal colonic enteric nervous system. For this, we investigated by immunocytochemistry the cellular localization of phosphorylated ERK1/2 (P-ERK) in a series of normal human colon samples removed from newborns and in patients with intestinal obstruction such as Hirschsprung's disease (HSCR), stenosis and atresia. We checked the presence of P-ERK in the three distinct histological layers of normal colon. Phosphorylated ERK was detected in the colonic mucosa, in the enteric nervous system and in endothelial cells. In the mucosa from normal colon, P-ERK was detected at the upper part of the crypt, while P-ERK activation in epithelial cells is altered in HSCR, stenosis and atresia. In the normal colon, strong P-ERK staining was detected in myenteric and submucosal enteric plexuses. Using confocal microscopy analyses, we observed that P-ERK staining was localized in enteric glial cells and not in enteric neurons. Strong P-ERK staining was also observed in plexuses from stenosis and atresia whereas in HSCR, hypertrophic nerve fibres were not stained.
Keywords
Colon/anatomy & histology, Colon/enzymology, Enteric Nervous System/anatomy & histology, Enteric Nervous System/enzymology, Enzyme Activation, Hirschsprung Disease/enzymology, Hirschsprung Disease/pathology, Humans, Infant, Infant, Newborn, Intestinal Atresia/enzymology, Intestinal Atresia/pathology, Intestinal Obstruction/enzymology, Intestinal Obstruction/pathology, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 3/metabolism, Phosphorylation
Pubmed
Create date
11/10/2011 10:30
Last modification date
20/08/2019 15:16
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