Chlamydia pneumoniae and Mycoplasma pneumoniae in children with cystic fibrosis: impact on bacterial respiratory microbiota diversity.

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State: Public
Version: Final published version
License: CC BY-NC 4.0
Serval ID
serval:BIB_ABE112806E5C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Chlamydia pneumoniae and Mycoplasma pneumoniae in children with cystic fibrosis: impact on bacterial respiratory microbiota diversity.
Journal
Pathogens and disease
Author(s)
Pittet L.F., Bertelli C., Scherz V., Rochat I., Mardegan C., Brouillet R., Jaton K., Mornand A., Kaiser L., Posfay-Barbe K., Asner S.A., Greub G.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Publication state
Published
Issued date
06/01/2021
Peer-reviewed
Oui
Volume
79
Number
1
Pages
ftaa074
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The contribution of intracellular and fastidious bacteria in Cystic fibrosis (CF) pulmonary exacerbations, and progressive lung function decline remains unknown. This project aimed to explore their impact on bacterial microbiota diversity over time in CF children.
Sixty-one children enrolled in the MUCOVIB multicentre prospective cohort provided 746 samples, mostly nasopharyngeal swabs, throat swabs and sputa which were analysed using culture, specific real-time qPCRs and 16S rRNA amplicon metagenomics.
Chlamydia pneumoniae (n = 3) and Mycoplasma pneumoniae (n = 1) were prospectively documented in 6.6% of CF children. Microbiota alpha-diversity in children with a documented C. pneumoniae was highly variable, similarly to children infected with Staphylococcus aureus or Pseudomonas aeruginosa. The transition from routine follow-up visits to pulmonary exacerbation (n = 17) yielded variable changes in diversity indexes with some extreme loss of diversity.
The high rate of C. pneumoniae detection supports the need for regular screenings in CF patients. A minor impact of C. pneumoniae on the microbial community structure was documented. Although detected in a single patient, M. pneumoniae should also be considered as a possible aetiology of lung infection in CF subjects.
Keywords
Biodiversity, Child, Child, Preschool, Chlamydophila Infections/microbiology, Chlamydophila pneumoniae/genetics, Chlamydophila pneumoniae/isolation & purification, Cystic Fibrosis/microbiology, DNA, Bacterial, Humans, Metagenomics, Microbiota, Mycoplasma pneumoniae/genetics, Mycoplasma pneumoniae/isolation & purification, Pneumonia, Mycoplasma/microbiology, Prospective Studies, RNA, Ribosomal, 16S, Respiratory System/microbiology, Sputum/microbiology, Chlamydiales, cystic fibrosis, intracellular bacteria, metagenomics, pneumonia, pulmonary exacerbation
Pubmed
Web of science
Open Access
Yes
Create date
07/12/2020 16:06
Last modification date
13/10/2021 6:42
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