Ppn2, a novel Zn2+-dependent polyphosphatase in the acidocalcisome-like yeast vacuole.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_ABA7A662D203
Type
Article: article from journal or magazin.
Collection
Publications
Title
Ppn2, a novel Zn2+-dependent polyphosphatase in the acidocalcisome-like yeast vacuole.
Journal
Journal of cell science
Author(s)
Gerasimaitė R., Mayer A.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Publication state
Published
Issued date
01/05/2017
Peer-reviewed
Oui
Volume
130
Number
9
Pages
1625-1636
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Acidocalcisome-like organelles are found in all kingdoms of life. Many of their functions, such as the accumulation and storage of metal ions, nitrogen and phosphate, the activation of blood clotting and inflammation, depend on the controlled synthesis and turnover of polyphosphate (polyP), a polymer of inorganic phosphate linked by phosphoric anhydride bonds. The exploration of the role of acidocalcisomes in metabolism and physiology requires the manipulation of polyP turnover, yet the complete set of proteins responsible for this turnover is unknown. Here, we identify a novel type of polyphosphatase operating in the acidocalcisome-like vacuoles of the yeast Saccharomyces cerevisiae, which we called Ppn2. Ppn2 belongs to the PPP-superfamily of metallophosphatases, is activated by Zn2+ ions and exclusively shows endopolyphosphatase activity. It is sorted to vacuoles via the multivesicular body pathway. Together with Ppn1, Ppn2 is responsible for a substantial fraction of polyphosphatase activity that is necessary to mobilize polyP stores, for example in response to phosphate scarcity. This finding opens the way to manipulating polyP metabolism more profoundly and deciphering its roles in phosphate and energy homeostasis, as well as in signaling.

Keywords
Acid Anhydride Hydrolases/chemistry, Acid Anhydride Hydrolases/metabolism, Acids/metabolism, Amino Acid Sequence, Gene Knockout Techniques, Multivesicular Bodies/metabolism, Polyphosphates/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae Proteins/chemistry, Saccharomyces cerevisiae Proteins/metabolism, Vacuoles/enzymology, Zinc/metabolism, Inorganic polyphosphate, Phosphate homeostasis, Polyphosphatase, Yeast vacuole
Pubmed
Web of science
Open Access
Yes
Create date
28/03/2017 17:28
Last modification date
20/08/2019 15:15
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