A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.

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Version: Final published version
Serval ID
serval:BIB_AB43CB71CC57
Type
Article: article from journal or magazin.
Collection
Publications
Title
A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
Journal
Journal of Cell Biology
Author(s)
Dudin O., Bendezú F.O., Groux R., Laroche T., Seitz A., Martin S.G.
ISSN
1540-8140 (Electronic)
ISSN-L
0021-9525
Publication state
Published
Issued date
2015
Volume
208
Number
7
Pages
897-911
Language
english
Abstract
Cell-cell fusion is essential for fertilization. For fusion of walled cells, the cell wall must be degraded at a precise location but maintained in surrounding regions to protect against lysis. In fission yeast cells, the formin Fus1, which nucleates linear actin filaments, is essential for this process. In this paper, we show that this formin organizes a specific actin structure-the actin fusion focus. Structured illumination microscopy and live-cell imaging of Fus1, actin, and type V myosins revealed an aster of actin filaments whose barbed ends are focalized near the plasma membrane. Focalization requires Fus1 and type V myosins and happens asynchronously always in the M cell first. Type V myosins are essential for fusion and concentrate cell wall hydrolases, but not cell wall synthases, at the fusion focus. Thus, the fusion focus focalizes cell wall dissolution within a broader cell wall synthesis zone to shift from cell growth to cell fusion.
Pubmed
Web of science
Open Access
Yes
Create date
28/04/2015 17:14
Last modification date
20/08/2019 15:15
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