Alzheimer disease pathology and the cerebrospinal fluid proteome.

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Version: Final published version
Serval ID
serval:BIB_AAE843C84F72
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Alzheimer disease pathology and the cerebrospinal fluid proteome.
Journal
Alzheimer's research & therapy
Author(s)
Dayon L., Núñez Galindo A., Wojcik J., Cominetti O., Corthésy J., Oikonomidi A., Henry H., Kussmann M., Migliavacca E., Severin I., Bowman G.L., Popp J.
ISSN
1758-9193 (Electronic)
Publication state
Published
Issued date
18/07/2018
Peer-reviewed
Oui
Volume
10
Number
1
Pages
66
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Altered proteome profiles have been reported in both postmortem brain tissues and body fluids of subjects with Alzheimer disease (AD), but their broad relationships with AD pathology, amyloid pathology, and tau-related neurodegeneration have not yet been fully explored. Using a robust automated MS-based proteomic biomarker discovery workflow, we measured cerebrospinal fluid (CSF) proteomes to explore their association with well-established markers of core AD pathology.
Cross-sectional analysis was performed on CSF collected from 120 older community-dwelling adults with normal (n = 48) or impaired cognition (n = 72). LC-MS quantified hundreds of proteins in the CSF. CSF concentrations of β-amyloid 1-42 (Aβ <sub>1-42</sub> ), tau, and tau phosphorylated at threonine 181 (P-tau181) were determined with immunoassays. First, we explored proteins relevant to biomarker-defined AD. Then, correlation analysis of CSF proteins with CSF markers of amyloid pathology, neuronal injury, and tau hyperphosphorylation (i.e., Aβ <sub>1-42</sub> , tau, P-tau181) was performed using Pearson's correlation coefficient and Bonferroni correction for multiple comparisons.
We quantified 790 proteins in CSF samples with MS. Four CSF proteins showed an association with CSF Aβ <sub>1-42</sub> levels (p value ≤ 0.05 with correlation coefficient (R) ≥ 0.38). We identified 50 additional CSF proteins associated with CSF tau and 46 proteins associated with CSF P-tau181 (p value ≤ 0.05 with R ≥ 0.37). The majority of those proteins that showed such associations were brain-enriched proteins. Gene Ontology annotation revealed an enrichment for synaptic proteins and proteins originating from reelin-producing cells and the myelin sheath.
We used an MS-based proteomic workflow to profile the CSF proteome in relation to cerebral AD pathology. We report strong evidence of previously reported CSF proteins and several novel CSF proteins specifically associated with amyloid pathology or neuronal injury and tau hyperphosphorylation.
Keywords
Age Factors, Aged, Aged, 80 and over, Alzheimer Disease/cerebrospinal fluid, Alzheimer Disease/genetics, Alzheimer Disease/pathology, Amyloid beta-Peptides/cerebrospinal fluid, Apolipoproteins E/genetics, Chromatography, Liquid, Educational Status, Female, Humans, Male, Middle Aged, Neuropsychological Tests, Peptide Fragments/cerebrospinal fluid, Phosphorylation, Proteome/metabolism, Sex Factors, Tandem Mass Spectrometry, tau Proteins/cerebrospinal fluid, Alzheimer disease, Amyloid, Biomarker, CSF, Cerebrospinal fluid, Mass spectrometry, Proteomics, Tandem mass tag, Tau
Pubmed
Web of science
Open Access
Yes
Create date
03/08/2018 16:11
Last modification date
20/08/2019 15:14
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