Stabilization of urinary biogenic amines measured in clinical chemistry laboratories.

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State: Public
Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_AADFBBB3E2CC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stabilization of urinary biogenic amines measured in clinical chemistry laboratories.
Journal
Clinica chimica acta; international journal of clinical chemistry
Author(s)
Eugster P.J., Centeno C., Dunand M., Seghezzi C., Grouzmann E.
ISSN
1873-3492 (Electronic)
ISSN-L
0009-8981
Publication state
Published
Issued date
03/2021
Peer-reviewed
Oui
Volume
514
Pages
24-28
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Urinary 5-hydroxyindoleacetic acid (5-HIAA), vanillylmandelic (VMA), homovanillic acid (HVA), catecholamines and metanephrines are produced in excess by catecholamine-producing tumors. These biogenic amines are unstable at low or high pH and require hydrochloric acid (HCl) to prevent their degradation. However, HCl addition may result in very low pH causing degradation or deconjugation of several metabolites. This study evaluated the buffering properties of sodium citrate to stabilize all biogenic amines. The metabolite concentrations were measured by LC-MS/MS or by a coulometric assay in 22 urine samples collected native and with HCl or sodium citrate. We studied the effect of pH, time (48 h, four weeks) and storage temperature at 22 °C, 4 °C, and -20 °C. We found that catecholamines degradation was prevented by HCl and citrate and that 5-HIAA was degraded in 5 out of 22 samples collected with HCl. All biogenic amines were efficiently stabilized by citrate for four weeks at 22 °C, except epinephrine (48 h at 4 °C, or four weeks at -20 °C). Sodium citrate did not cause quantification or analytical artefacts concerns. In conclusion, sodium citrate is a non-hazardous alternative to HCl for patients to send unfrozen urine samples to the laboratory which may safely store the sample for four weeks.
Keywords
Clinical Biochemistry, Biochemistry, Biochemistry, medical, General Medicine, 5-hydroxyindoleacetic acid, catecholamines, metanephrines, stability, urine, vanillylmandelic acid, Catecholamines, Metanephrines, Stability, Urine, Vanillylmandelic acid
Pubmed
Web of science
Open Access
Yes
Create date
18/12/2020 10:32
Last modification date
13/03/2021 7:22
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