Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis

Details

Serval ID
serval:BIB_AAB2B8C7C9E3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis
Journal
Journal of Histochemistry and Cytochemistry
Author(s)
Ishida-Yamamoto  A., Eady  R. A., Watt  F. M., Roop  D. R., Hohl  D., Iizuka  H.
ISSN
0022-1554 (Print)
Publication state
Published
Issued date
02/1996
Volume
44
Number
2
Pages
167-75
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
The cornified cell envelope (CE) is an insoluble, highly resistant structure formed beneath the plasma membrane of differentiating keratinocytes. It consists of various crosslinked precursor proteins, including involucrin and loricrin. However, neither the normal assembly process of CE nor its alteration in skin disorders has been fully characterized. In this study we analyzed CE formation in normal skin and in lesional psoriatic skin by immunoelectron microscopy. In the superficial granular cells of normal epidermis, involucrin labeling was detected along the plasma membrane, whereas loricrin staining was mostly distributed diffusely within the cells, occasionally with some additional granular aggregates within the cytoplasm and nucleus. Loricrin was also present predominantly on the desmosomal attachment plaques, colocalizing with desmoglein. In the cornified cells, involucrin labeling was reduced, whereas loricrin labeling was retained and continuously decorated the CEs, with relative sparing of the desmosomal areas. In typical psoriatic epidermis, involucrin staining was very intense but loricrin labeling was markedly reduced. The involucrin-positive CEs were formed precociously and further maturation of CE did not occur. These results suggest that formation of CE occurs sequentially, initially involving involucrin deposition and subsequently involving loricrin incorporation. Psoriatic epidermis demonstrates a lack of proper CE maturation.
Keywords
Aged Aged, 80 and over Animals Cell Membrane/immunology/*ultrastructure Cytoskeletal Proteins/analysis/immunology Desmogleins Desmoplakins Epidermis/pathology/*ultrastructure Female Humans Intermediate Filament Proteins/analysis/immunology Keratinocytes/pathology/ultrastructure Male Membrane Proteins/analysis/immunology Mice Microscopy, Immunoelectron Middle Aged Protein Precursors/analysis/immunology Psoriasis/immunology/*pathology Rabbits
Pubmed
Web of science
Create date
25/01/2008 16:35
Last modification date
20/08/2019 15:14
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