In vivo dual RNA-seq reveals that neutrophil recruitment underlies differential tissue tropism of Streptococcus pneumoniae.

Details

Serval ID
serval:BIB_AA4F7869B18E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vivo dual RNA-seq reveals that neutrophil recruitment underlies differential tissue tropism of Streptococcus pneumoniae.
Journal
Communications biology
Author(s)
Minhas V., Aprianto R., McAllister L.J., Wang H., David S.C., McLean K.T., Comerford I., McColl S.R., Paton J.C., Veening J.W. (co-last), Trappetti C.
ISSN
2399-3642 (Electronic)
ISSN-L
2399-3642
Publication state
Published
Issued date
05/06/2020
Peer-reviewed
Oui
Volume
3
Number
1
Pages
293
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Streptococcus pneumoniae is a genetically diverse human-adapted pathogen commonly carried asymptomatically in the nasopharynx. We have recently shown that a single nucleotide polymorphism (SNP) in the raffinose pathway regulatory gene rafR accounts for a difference in the capacity of clonally-related strains to cause localised versus systemic infection. Using dual RNA-seq, we show that this SNP affects expression of bacterial genes encoding multiple sugar transporters, and fine-tunes carbohydrate metabolism, along with extensive rewiring of host transcriptional responses to infection, particularly expression of genes encoding cytokine and chemokine ligands and receptors. The data predict a crucial role for differential neutrophil recruitment (confirmed by in vivo neutrophil depletion and IL-17 neutralization) indicating that early detection of bacteria by the host in the lung environment is crucial for effective clearance. Thus, dual RNA-seq provides a powerful tool for understanding complex host-pathogen interactions and reveals how a single bacterial SNP can drive differential disease outcomes.
Pubmed
Web of science
Open Access
Yes
Create date
10/06/2020 19:26
Last modification date
30/10/2020 6:23
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